Start with the handles already present on the sample and the handle or partner to connect. Distinguish click-handle installation, amine–thiol bridging, thiol modification and biotin labeling.
20 products
Filter by reaction handles and conjugation task, then check the spacer and pack size. See methods for reagent-specific partners, reaction order and purification.
20 matching products
| Product / Cat. No. | Product form | Pack sizes | Product details |
|---|---|---|---|
| (4E)-TCO-NHS EsterQH-70-7011 | NHS Ester | 50 mg / 100 mg / 250 mg | |
| (4E)-TCO-PEG4-NHS EsterQH-70-7012 | NHS Ester | 50 mg / 100 mg / 250 mg | |
| Biotin-SS-Sulfo-NHS EsterQH-70-7019 | NHS Ester | 50 mg / 100 mg / 250 mg | |
| Cyclooctyne-O-NHS EsterQH-70-7016 | NHS Ester | 50 mg / 100 mg / 250 mg | |
| DBCO-PEG4-NHS EsterQH-70-7014 | NHS Ester | 50 mg / 100 mg / 250 mg | |
| Methyltetrazine PEG4 NHS EsterQH-70-7003 | NHS Ester | 50 mg / 100 mg / 250 mg | |
| N3-C5-NHS EsterQH-70-7013 | NHS Ester | 50 mg / 100 mg / 250 mg | |
| TCO NHS EsterQH-70-7001 | NHS Ester | 50 mg / 100 mg / 250 mg | |
| Tetrazine NHS EsterQH-70-7009 | NHS Ester | 50 mg / 100 mg / 250 mg | |
| Tetrazine-PEG5-NHS EsterQH-70-7010 | NHS Ester | 50 mg / 100 mg / 250 mg | |
| N-Ethylmaleimide(NEM)QH-70-7006 | Maleimide | 50 mg / 100 mg / 250 mg | |
| Traut's ReagentQH-70-7004 | Thiolation | 50 mg / 100 mg / 250 mg | |
| BIOTIN-XX Tyramide reagentQH-70-7002 | Tyramide | 50 mg / 100 mg / 250 mg | |
| 1-Methylcyclopropene-PEG4-COOHQH-70-7018 | Methylcyclopropene/Free acid | 50 mg / 100 mg / 250 mg | |
| Azide Acetic AcidQH-70-7020 | Azide/Free acid | 50 mg / 100 mg / 250 mg | |
| exo BCN-O-PNBQH-70-7015 | BCN/PNB carbonate | 50 mg / 100 mg / 250 mg | |
| SMCCQH-70-7005 | NHS Ester/Maleimide | 50 mg / 100 mg / 250 mg | |
| SPDP CrosslinkersQH-70-7007 | NHS Ester/Pyridyldithiol | 50 mg / 100 mg / 250 mg | |
| Sulfo-SMCCQH-70-7008 | NHS Ester/Maleimide | 50 mg / 100 mg / 250 mg | |
| Tetrazine-PEG4-amineHCl saltQH-70-7017 | Tetrazine/NH2 | 50 mg / 100 mg / 250 mg |
For support, include: Existing handles on components A/B, intended linkage and any need for cleavage
Identify which linker end reacts with the existing sample and which is retained for the next step; a click-handle name does not make every molecular pair compatible.
The maleimide end of SMCC/sulfo-SMCC gives a thioether-containing linkage with thiols; SPDP forms a reducible disulfide through thiol exchange.
These reagents serve opposite purposes and are not substitutes within the same coupling step.
Biotin-SS-sulfo-NHS and biotin-XX tyramide require different reaction targets and components.
Primary amines through activation; subsequently a suitable alkyne
Introduce a small azide handle through carboxyl activation for subsequent click conjugation.
View this protocolPrimary amines; subsequently biotin-affinity detection
Install a biotin tag with a reducible disulfide onto primary-amine-bearing molecules.
View this protocolPrimary amines through activation; subsequently a matched tetrazine
Activate the carboxyl to couple an amine-bearing molecule, retaining methylcyclopropene for a matched tetrazine reaction.
View this protocolActivated carboxyls; subsequently a suitable TCO
Couple the free amine to an activated carboxyl while retaining tetrazine for subsequent TCO ligation.
View this protocolPrimary amines; subsequently an azide
Use an activated ester to install cyclooctyne on amine-bearing samples for azide coupling.
View this protocolPrimary amines; subsequently a suitable azide
Install the strained alkyne BCN on amine-bearing molecules through an activated carbonate.
View this protocolPrimary amines; subsequently an azide
Install DBCO–PEG4 on primary-amine-bearing molecules through NHS coupling for subsequent copper-free click ligation.
View this protocolPrimary amines; subsequently a suitable alkyne
Introduce an azide handle onto primary-amine-bearing molecules through an NHS ester.
View this protocolPrimary amines; subsequently a suitable tetrazine
Install (4E)-TCO on amine-bearing samples through a PEG4 spacer.
View this protocolPrimary amines; subsequently a suitable tetrazine
Install a (4E)-TCO handle on amine-bearing samples for subsequent tetrazine ligation.
View this protocolPrimary amines; subsequently a matched TCO
Introduce tetrazine through a PEG5-spaced NHS reagent for sequential bioconjugation.
View this protocolPrimary amines; subsequently a matched TCO
Install tetrazine handles on primary-amine-bearing molecules through an NHS ester.
View this protocolPrimary amines and free thiols
Connect amine- and free-thiol-bearing samples through sulfo-NHS and maleimide ends.
View this protocolPrimary amines and free thiols
Connect primary amines and free thiols through NHS and pyridyl-disulfide groups, forming a reducible linkage.
View this protocolAccessible free thiols
Block accessible free thiols to control residual thiol reactivity during sample processing.
View this protocolPrimary amines and free thiols
Connect primary-amine- and free-thiol-bearing components through NHS and maleimide ends.
View this protocolAccessible primary amines
Convert accessible sample primary amines into thiol-containing handles for subsequent coupling.
View this protocolPrimary amines; subsequently a suitable TCO
Install a methyltetrazine–PEG4 handle on primary-amine-bearing molecules through the NHS end.
View this protocolNearby deposition sites in a suitable HRP-localized system
A biotinylated tyramide substrate for enzyme-catalyzed local deposition followed by biotin-affinity detection.
View this protocolPrimary amines; subsequently a suitable tetrazine
Install TCO on primary-amine-bearing molecules for subsequent tetrazine ligation.
View this protocolUse CuAAC for terminal alkynes and SPAAC for strained alkynes. Identify the handle before assessing copper compatibility.
Applies to: Only for azide/alkyne linkers; consult the separate references for TCO/tetrazine and cyclopropene chemistry.
These papers and manufacturer methods are selected for the current product use. Their reagents, conditions and parameters belong to the reported systems, not to measured Qihe product performance or universal operating conditions.
thermofisher.com
Distinguishes amine-reactive forms such as NHS and isothiocyanate and competing buffers; separately establish whether a nucleic-acid sample has accessible amines.
thermofisher.com
Explains thiol addition, blocking and disulfide exchange; maleimide, NEM, SPDP and Traut’s reagent have different purposes and products.
Thermo Fisher Scientific · SMCC and Sulfo-SMCC · MAN0011295 Rev. C.0
Distinguishes the partners and reaction order of the NHS and maleimide ends and removal of free linker, supporting bridging routes for NH2 dyes.
Shafer DE, Inman JK, Lees A. · Analytical Biochemistry · 282(1):161–164 · 2000
Examines TCEP interactions with reactive groups, informing evaluation of reductant carryover and buffer exchange.
Thermo Fisher Scientific
Sample preparation, free biotin and background control in biotin-affinity detection.
Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ. · Journal of Immunological Methods · 125(1–2):279–285 · 1989
Introduces enzyme-catalyzed reporter deposition for signal amplification, informing enzyme-dependence and background controls.
Blackman ML, Royzen M, Fox JM. · Journal of the American Chemical Society · 130(41):13518–13519 · 2008
Studies catalyst-free tetrazine–TCO cycloaddition. Rates and stability depend on substituent structure; reported kinetics are not specifications for every linker.
DOI: 10.1021/ja8053805
Yang J, Liang Y, Šečkutė J, Houk KN, Devaraj NK. · Chemistry – A European Journal · 20(12):3365–3375 · 2014
Compares substituent effects on methylcyclopropene reactivity and aqueous stability, supporting evaluation of the actual reaction pair.
Agard NJ, Prescher JA, Bertozzi CR. · Journal of the American Chemical Society · 126(46):15046–15047 · 2004
Introduces ring-strain-driven azide–alkyne coupling without copper, a foundational study in SPAAC bioconjugation.
DOI: 10.1021/ja044996f
Presolski SI, Hong VP, Finn MG. · Current Protocols in Chemical Biology · 3(4):153–162 · 2011
Describes CuAAC catalyst systems and reaction assessment for terminal-alkyne substrates.
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Handling, storage and emergency response information.
First identify existing handles on components A and B and the intended retained handle or cleavable bond, then compare spacers. PEG length changes spacing but does not by itself guarantee aqueous solubility or a fixed reaction rate.
Separate handle installation from final ligation. React the reagent’s other end with the sample and purify, then pair DBCO/BCN with azide, terminal alkyne with azide, or TCO with a suitable tetrazine. These use SPAAC, CuAAC or a distinct ligation with different conditions.
SMCC maleimide gives a thioether-containing thiol linkage, while SPDP forms a reducible disulfide through thiol exchange. Select for the intended reductive release. Complete and purify NHS-end modification before coupling the second component’s free thiols.
Traut’s reagent introduces thiol-bearing handles from suitable primary amines; NEM caps existing free thiols. Complete any required thiol coupling before considering capping, then remove excess reagent.
Biotin-SS-sulfo-NHS labels primary amines through a reducible spacer. Biotin-XX tyramide supports local deposition in a suitable HRP/peroxide system, requiring endogenous-peroxidase and deposition-background control rather than an NHS-coupling procedure.
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