Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
BIOTIN-XX Tyramide reagent
Cat. No.QH-70-7002
A biotinylated tyramide substrate for enzyme-catalyzed local deposition followed by biotin-affinity detection.
Packs & quoteDefine the sequence
Validate at small scale
Separate and proceed
Product formTyramide
TargetNearby deposition sites in a suitable HRP-localized system
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Enzyme-catalyzed deposition
With suitable peroxidase and peroxide conditions, tyramide forms reactive intermediates that deposit biotin near the enzyme. It is not an NHS biotinylation reagent or a complete TSA detection kit.
Biotin signal amplification
A biotinylated tyramide substrate for enzyme-catalyzed local deposition followed by biotin-affinity detection.
Product and functional assessment
After reaction and washes, detect biotin with a matched streptavidin system and include a no-HRP control.
Specifications
Product information
- Product name
- BIOTIN-XX Tyramide reagent
- Cat. No.
- QH-70-7002
- Product form
- Tyramide
- Target
- Nearby deposition sites in a suitable HRP-localized system
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 851113-28-5
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Address endogenous peroxidase and biotin background, then verify enzyme localization and deposition time.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Establish HRP localization and background control before adding the compatible deposition substrate system.
Validate at small scale
Address endogenous peroxidase and biotin background, then verify enzyme localization and deposition time.
Separate and proceed
Stop deposition and wash before biotin-affinity detection, controlling background from excessive deposition.
Assess the outcome
After reaction and washes, detect biotin with a matched streptavidin system and include a no-HRP control.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
BIOTIN-XX Tyramide reagentQH-70-7002
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
No-enzyme and no-primary controls
Assess enzyme-dependent deposition, nonspecific localization and endogenous background.
Reaction-pair control
Omit activation or a paired component to assess adsorption, residual reagents and nonspecific reaction.
References
Thermo Fisher · Biotin binding and interference
Thermo Fisher Scientific
Sample preparation, free biotin and background control in biotin-affinity detection.
Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.
Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ. · Journal of Immunological Methods · 125(1–2):279–285 · 1989
Introduces enzyme-catalyzed reporter deposition for signal amplification, informing enzyme-dependence and background controls.
Frequently asked questions
Can it be used as an NHS biotinylation reagent?
With suitable peroxidase and peroxide conditions, tyramide forms reactive intermediates that deposit biotin near the enzyme. It is not an NHS biotinylation reagent or a complete TSA detection kit.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
After reaction and washes, detect biotin with a matched streptavidin system and include a no-HRP control.