Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
Biotin-SS-Sulfo-NHS Ester
Cat. No.QH-70-7019
Install a biotin tag with a reducible disulfide onto primary-amine-bearing molecules.
Packs & quoteBiotin
Affinity handleLinker
NHS
Amine-reactive endProduct formNHS Ester
TargetPrimary amines; subsequently biotin-affinity detection
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Reactive ends and coupling route
The sulfo-NHS end forms an amide with an amine. A spacer disulfide can be cleaved under suitable reducing conditions. Validate capture, release or surface-labeling use for the sample.
Sequential bioconjugation
Install a biotin tag with a reducible disulfide onto primary-amine-bearing molecules.
Product and functional assessment
Assess biotin availability and reductive release separately, recording effects on sample disulfides.
Specifications
Product information
- Product name
- Biotin-SS-Sulfo-NHS Ester
- Cat. No.
- QH-70-7019
- Product form
- NHS Ester
- Target
- Primary amines; subsequently biotin-affinity detection
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 325143-98-4
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Avoid competing amines and unsuitable reductants before labeling; remove free biotin reagent before affinity detection.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Confirm the first target group and subsequent partner, then set input, pH, temperature and time for this reagent.
Validate at small scale
Avoid competing amines and unsuitable reductants before labeling; remove free biotin reagent before affinity detection.
Separate and proceed
Remove free reagents that interfere with the next step, retain the desired handle and complete the planned pairing.
Assess the outcome
Assess biotin availability and reductive release separately, recording effects on sample disulfides.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
Biotin-SS-Sulfo-NHS EsterQH-70-7019
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untreated sample
Compare integrity and function with an untreated aliquot from the same preparation.
Reaction-pair control
Omit activation or a paired component to assess adsorption, residual reagents and nonspecific reaction.
References
Thermo Fisher · Biotin binding and interference
Thermo Fisher Scientific
Sample preparation, free biotin and background control in biotin-affinity detection.
Thermo Fisher · Amine-reactive chemistry
thermofisher.com
Distinguishes amine-reactive forms such as NHS and isothiocyanate and competing buffers; separately establish whether a nucleic-acid sample has accessible amines.
Frequently asked questions
How do this reagent’s handles participate in coupling?
The sulfo-NHS end forms an amide with an amine. A spacer disulfide can be cleaved under suitable reducing conditions. Validate capture, release or surface-labeling use for the sample.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
Assess biotin availability and reductive release separately, recording effects on sample disulfides.