Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
DBCO-PEG4-NHS Ester
Cat. No.QH-70-7014
Install DBCO–PEG4 on primary-amine-bearing molecules through NHS coupling for subsequent copper-free click ligation.
Packs & quoteDBCO
Azide-reactive endPEG4
NHS
Amine-reactive endProduct formNHS Ester
TargetPrimary amines; subsequently an azide
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Reactive ends and coupling route
NHS couples to an amine while retaining strained-alkyne DBCO for SPAAC with an azide. This linker installs DBCO; it is not a DBCO fluorophore and does not directly provide a fluorescence readout.
Sequential bioconjugation
Install DBCO–PEG4 on primary-amine-bearing molecules through NHS coupling for subsequent copper-free click ligation.
Product and functional assessment
After purification, confirm DBCO availability with an azide partner and exclude background from unreacted linker.
Specifications
Product information
- Product name
- DBCO-PEG4-NHS Ester
- Cat. No.
- QH-70-7014
- Product form
- NHS Ester
- Target
- Primary amines; subsequently an azide
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 1427004-19-0
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Avoid competing amines during NHS coupling and review free azide reagents and sodium azide before the DBCO step.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Confirm the first target group and subsequent partner, then set input, pH, temperature and time for this reagent.
Validate at small scale
Avoid competing amines during NHS coupling and review free azide reagents and sodium azide before the DBCO step.
Separate and proceed
Remove free reagents that interfere with the next step, retain the desired handle and complete the planned pairing.
Assess the outcome
After purification, confirm DBCO availability with an azide partner and exclude background from unreacted linker.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
DBCO-PEG4-NHS EsterQH-70-7014
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untreated sample
Compare integrity and function with an untreated aliquot from the same preparation.
Reaction-pair control
Omit activation or a paired component to assess adsorption, residual reagents and nonspecific reaction.
Technical articles
Application support
Include your sample, formulation and detection method when requesting application guidance.
References
Thermo Fisher · Amine-reactive chemistry
thermofisher.com
Distinguishes amine-reactive forms such as NHS and isothiocyanate and competing buffers; separately establish whether a nucleic-acid sample has accessible amines.
A Strain-Promoted [3 + 2] Azide–Alkyne Cycloaddition for Covalent Modification of Biomolecules in Living Systems
Agard NJ, Prescher JA, Bertozzi CR. · Journal of the American Chemical Society · 126(46):15046–15047 · 2004
Introduces ring-strain-driven azide–alkyne coupling without copper, a foundational study in SPAAC bioconjugation.
DOI: 10.1021/ja044996f
Frequently asked questions
How do this reagent’s handles participate in coupling?
NHS couples to an amine while retaining strained-alkyne DBCO for SPAAC with an azide. This linker installs DBCO; it is not a DBCO fluorophore and does not directly provide a fluorescence readout.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
After purification, confirm DBCO availability with an azide partner and exclude background from unreacted linker.