Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
exo BCN-O-PNB
Cat. No.QH-70-7015
Install the strained alkyne BCN on amine-bearing molecules through an activated carbonate.
Packs & quoteDefine the sequence
Validate at small scale
Separate and proceed
Product formBCN/PNB carbonate
TargetPrimary amines; subsequently a suitable azide
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Reactive ends and coupling route
The PNB-activated carbonate couples to an amine while retaining BCN for subsequent copper-free azide coupling. The activated end is not NHS; do not transfer NHS solvation or timing without validation.
Sequential bioconjugation
Install the strained alkyne BCN on amine-bearing molecules through an activated carbonate.
Product and functional assessment
Remove free reagent and leaving-group products before assessing BCN coupling.
Specifications
Product information
- Product name
- exo BCN-O-PNB
- Cat. No.
- QH-70-7015
- Product form
- BCN/PNB carbonate
- Target
- Primary amines; subsequently a suitable azide
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 1380006-72-3
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Check moisture sensitivity and the required solvent for the activated carbonate.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Confirm the first target group and subsequent partner, then set input, pH, temperature and time for this reagent.
Validate at small scale
Check moisture sensitivity and the required solvent for the activated carbonate.
Separate and proceed
Remove free reagents that interfere with the next step, retain the desired handle and complete the planned pairing.
Assess the outcome
Remove free reagent and leaving-group products before assessing BCN coupling.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
exo BCN-O-PNBQH-70-7015
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untreated sample
Compare integrity and function with an untreated aliquot from the same preparation.
Reaction-pair control
Omit activation or a paired component to assess adsorption, residual reagents and nonspecific reaction.
Technical articles
Application support
Include your sample, formulation and detection method when requesting application guidance.
References
A Strain-Promoted [3 + 2] Azide–Alkyne Cycloaddition for Covalent Modification of Biomolecules in Living Systems
Agard NJ, Prescher JA, Bertozzi CR. · Journal of the American Chemical Society · 126(46):15046–15047 · 2004
Introduces ring-strain-driven azide–alkyne coupling without copper, a foundational study in SPAAC bioconjugation.
DOI: 10.1021/ja044996f
Frequently asked questions
How do this reagent’s handles participate in coupling?
The PNB-activated carbonate couples to an amine while retaining BCN for subsequent copper-free azide coupling. The activated end is not NHS; do not transfer NHS solvation or timing without validation.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
Remove free reagent and leaving-group products before assessing BCN coupling.