Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
Sulfo-SMCC
Cat. No.QH-70-7008
Connect amine- and free-thiol-bearing samples through sulfo-NHS and maleimide ends.
Packs & quoteDefine the sequence
Validate at small scale
Separate and proceed
Product formNHS Ester/Maleimide
TargetPrimary amines and free thiols
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Reactive ends and coupling route
Sulfo-NHS acylates an amine while maleimide is retained for thiol coupling. Solvent selection for the sulfonated form differs from SMCC; prepare according to this reagent.
Sequential bioconjugation
Connect amine- and free-thiol-bearing samples through sulfo-NHS and maleimide ends.
Product and functional assessment
Assess product distribution and function and confirm removal of free linker that could consume the next component.
Specifications
Product information
- Product name
- Sulfo-SMCC
- Cat. No.
- QH-70-7008
- Product form
- NHS Ester/Maleimide
- Target
- Primary amines and free thiols
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 92921-24-9
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Plan step-specific buffers, avoiding competing amines initially and free thiols or reductants during the second step.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Confirm the first target group and subsequent partner, then set input, pH, temperature and time for this reagent.
Validate at small scale
Plan step-specific buffers, avoiding competing amines initially and free thiols or reductants during the second step.
Separate and proceed
Remove free reagents that interfere with the next step, retain the desired handle and complete the planned pairing.
Assess the outcome
Assess product distribution and function and confirm removal of free linker that could consume the next component.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
Sulfo-SMCCQH-70-7008
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untreated sample
Compare integrity and function with an untreated aliquot from the same preparation.
Reaction-pair control
Omit activation or a paired component to assess adsorption, residual reagents and nonspecific reaction.
References
Thermo Fisher · Amine-reactive chemistry
thermofisher.com
Distinguishes amine-reactive forms such as NHS and isothiocyanate and competing buffers; separately establish whether a nucleic-acid sample has accessible amines.
Thermo Fisher · Sulfhydryl-reactive chemistry
thermofisher.com
Explains thiol addition, blocking and disulfide exchange; maleimide, NEM, SPDP and Traut’s reagent have different purposes and products.
Stepwise amine-to-thiol coupling with SMCC
Thermo Fisher Scientific · SMCC and Sulfo-SMCC · MAN0011295 Rev. C.0
Distinguishes the partners and reaction order of the NHS and maleimide ends and removal of free linker, supporting bridging routes for NH2 dyes.
Reaction of Tris(2-carboxyethyl)phosphine (TCEP) with maleimide and alpha-haloacyl groups: anomalous elution of TCEP by gel filtration
Shafer DE, Inman JK, Lees A. · Analytical Biochemistry · 282(1):161–164 · 2000
Examines TCEP interactions with reactive groups, informing evaluation of reductant carryover and buffer exchange.
Frequently asked questions
How do this reagent’s handles participate in coupling?
Sulfo-NHS acylates an amine while maleimide is retained for thiol coupling. Solvent selection for the sulfonated form differs from SMCC; prepare according to this reagent.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
Assess product distribution and function and confirm removal of free linker that could consume the next component.