Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
Tetrazine-PEG4-amineHCl salt
Cat. No.QH-70-7017
Couple the free amine to an activated carboxyl while retaining tetrazine for subsequent TCO ligation.
Packs & quoteDefine the sequence
Validate at small scale
Separate and proceed
Product formTetrazine/NH2
TargetActivated carboxyls; subsequently a suitable TCO
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Reactive ends and coupling route
This reagent supplies tetrazine and an amine salt, not an NHS ester. Couple the amine to a suitable activated carboxyl; retained tetrazine supports inverse-electron-demand Diels–Alder ligation.
Sequential bioconjugation
Couple the free amine to an activated carboxyl while retaining tetrazine for subsequent TCO ligation.
Product and functional assessment
Verify tetrazine availability and target function; the amine end is not an amine-reactive ester.
Specifications
Product information
- Product name
- Tetrazine-PEG4-amineHCl salt
- Cat. No.
- QH-70-7017
- Product form
- Tetrazine/NH2
- Target
- Activated carboxyls; subsequently a suitable TCO
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 2752945-36-9
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Account for the salt form and pH, selecting activation conditions that support amine coupling while preserving tetrazine.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Confirm the first target group and subsequent partner, then set input, pH, temperature and time for this reagent.
Validate at small scale
Account for the salt form and pH, selecting activation conditions that support amine coupling while preserving tetrazine.
Separate and proceed
Remove free reagents that interfere with the next step, retain the desired handle and complete the planned pairing.
Assess the outcome
Verify tetrazine availability and target function; the amine end is not an amine-reactive ester.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
Tetrazine-PEG4-amineHCl saltQH-70-7017
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untreated sample
Compare integrity and function with an untreated aliquot from the same preparation.
Reaction-pair control
Omit activation or a paired component to assess adsorption, residual reagents and nonspecific reaction.
Frequently asked questions
How do this reagent’s handles participate in coupling?
This reagent supplies tetrazine and an amine salt, not an NHS ester. Couple the amine to a suitable activated carboxyl; retained tetrazine supports inverse-electron-demand Diels–Alder ligation.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
Verify tetrazine availability and target function; the amine end is not an amine-reactive ester.