Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
Tetrazine NHS Ester
Cat. No.QH-70-7009
Install tetrazine handles on primary-amine-bearing molecules through an NHS ester.
Packs & quoteTetrazine
TCO-reactive endLinker
NHS
Amine-reactive endProduct formNHS Ester
TargetPrimary amines; subsequently a matched TCO
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Reactive ends and coupling route
First couple the NHS ester to sample amines, then use the retained tetrazine for TCO cycloaddition. Evaluate compatibility, purification and function for both steps.
Sequential bioconjugation
Install tetrazine handles on primary-amine-bearing molecules through an NHS ester.
Product and functional assessment
Verify available tetrazine after the first cleanup and include a no-TCO control.
Specifications
Product information
- Product name
- Tetrazine NHS Ester
- Cat. No.
- QH-70-7009
- Product form
- NHS Ester
- Target
- Primary amines; subsequently a matched TCO
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 1616668-55-3
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Confirm solvent and tetrazine stability; do not use another tetrazine structure’s rate as this product’s specification.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Confirm the first target group and subsequent partner, then set input, pH, temperature and time for this reagent.
Validate at small scale
Confirm solvent and tetrazine stability; do not use another tetrazine structure’s rate as this product’s specification.
Separate and proceed
Remove free reagents that interfere with the next step, retain the desired handle and complete the planned pairing.
Assess the outcome
Verify available tetrazine after the first cleanup and include a no-TCO control.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
Tetrazine NHS EsterQH-70-7009
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untreated sample
Compare integrity and function with an untreated aliquot from the same preparation.
Reaction-pair control
Omit activation or a paired component to assess adsorption, residual reagents and nonspecific reaction.
References
Thermo Fisher · Amine-reactive chemistry
thermofisher.com
Distinguishes amine-reactive forms such as NHS and isothiocyanate and competing buffers; separately establish whether a nucleic-acid sample has accessible amines.
Tetrazine ligation: fast bioconjugation based on inverse-electron-demand Diels–Alder reactivity
Blackman ML, Royzen M, Fox JM. · Journal of the American Chemical Society · 130(41):13518–13519 · 2008
Studies catalyst-free tetrazine–TCO cycloaddition. Rates and stability depend on substituent structure; reported kinetics are not specifications for every linker.
DOI: 10.1021/ja8053805
Frequently asked questions
How do this reagent’s handles participate in coupling?
First couple the NHS ester to sample amines, then use the retained tetrazine for TCO cycloaddition. Evaluate compatibility, purification and function for both steps.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
Verify available tetrazine after the first cleanup and include a no-TCO control.