Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
N3-C5-NHS Ester
Cat. No.QH-70-7013
Introduce an azide handle onto primary-amine-bearing molecules through an NHS ester.
Packs & quoteN₃
Matched-alkyne endLinker
NHS
Amine-reactive endProduct formNHS Ester
TargetPrimary amines; subsequently a suitable alkyne
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Reactive ends and coupling route
NHS forms an amide with a primary amine, retaining azide for click coupling. Pair terminal alkynes through CuAAC or suitable strained alkynes such as DBCO through SPAAC.
Sequential bioconjugation
Introduce an azide handle onto primary-amine-bearing molecules through an NHS ester.
Product and functional assessment
Choose the next reaction system for the alkyne type and include a nonazidated sample control.
Specifications
Product information
- Product name
- N3-C5-NHS Ester
- Cat. No.
- QH-70-7013
- Product form
- NHS Ester
- Target
- Primary amines; subsequently a suitable alkyne
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 866363-70-4
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Remove free azide modification reagent so it does not consume the next alkyne reagent.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Confirm the first target group and subsequent partner, then set input, pH, temperature and time for this reagent.
Validate at small scale
Remove free azide modification reagent so it does not consume the next alkyne reagent.
Separate and proceed
Remove free reagents that interfere with the next step, retain the desired handle and complete the planned pairing.
Assess the outcome
Choose the next reaction system for the alkyne type and include a nonazidated sample control.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
N3-C5-NHS EsterQH-70-7013
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untreated sample
Compare integrity and function with an untreated aliquot from the same preparation.
Reaction-pair control
Omit activation or a paired component to assess adsorption, residual reagents and nonspecific reaction.
Technical articles
Application support
Include your sample, formulation and detection method when requesting application guidance.
References
Thermo Fisher · Amine-reactive chemistry
thermofisher.com
Distinguishes amine-reactive forms such as NHS and isothiocyanate and competing buffers; separately establish whether a nucleic-acid sample has accessible amines.
A Strain-Promoted [3 + 2] Azide–Alkyne Cycloaddition for Covalent Modification of Biomolecules in Living Systems
Agard NJ, Prescher JA, Bertozzi CR. · Journal of the American Chemical Society · 126(46):15046–15047 · 2004
Introduces ring-strain-driven azide–alkyne coupling without copper, a foundational study in SPAAC bioconjugation.
DOI: 10.1021/ja044996f
Copper-Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation
Presolski SI, Hong VP, Finn MG. · Current Protocols in Chemical Biology · 3(4):153–162 · 2011
Describes CuAAC catalyst systems and reaction assessment for terminal-alkyne substrates.
Frequently asked questions
How do this reagent’s handles participate in coupling?
NHS forms an amide with a primary amine, retaining azide for click coupling. Pair terminal alkynes through CuAAC or suitable strained alkynes such as DBCO through SPAAC.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
Choose the next reaction system for the alkyne type and include a nonazidated sample control.