Protein labeling calculator
Calculate the dye amount and expected degree of labeling (DOL) for NHS ester fluorescent dye conjugation
Input parameters
Reference: Cy5-NHS ≈ 792, FITC ≈ 389
Typically 10 mM in DMSO
Recommended 5–20×
Results
Fill in the parameters and click "Calculate" to see results
Calculation principles
Protein amount (μmol) = concentration (mg/mL) × volume (μL) / MW (Da)
Dye required (μmol) = protein amount (μmol) × molar ratio
Dye stock volume (μL) = dye required (μmol) / stock concentration (mM) × 1000
Note: actual DOL is affected by buffer pH, reaction time, protein activity and other factors. Use the UV-Vis measured value as the definitive result.