Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
Traut's Reagent
Cat. No.QH-70-7004
Convert accessible sample primary amines into thiol-containing handles for subsequent coupling.
Packs & quoteDefine the sequence
Validate at small scale
Separate and proceed
Product formThiolation
TargetAccessible primary amines
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Thiol introduction
Traut’s reagent (2-iminothiolane) introduces thiols and is not a fluorophore. Newly installed thiols can oxidize, so assess them promptly and proceed to the next reaction.
Subsequent thiol coupling
Convert accessible sample primary amines into thiol-containing handles for subsequent coupling.
Product and functional assessment
Assess available thiols before coupling to a matched reagent such as maleimide.
Specifications
Product information
- Product name
- Traut's Reagent
- Cat. No.
- QH-70-7004
- Product form
- Thiolation
- Target
- Accessible primary amines
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 4781-83-3
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Avoid competing amines, control modification extent and remove unreacted reagent.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Treat target amines in a compatible buffer and compare modification extent with sample integrity.
Validate at small scale
Avoid competing amines, control modification extent and remove unreacted reagent.
Separate and proceed
Remove free reagents that interfere with the next step, retain the desired handle and complete the planned pairing.
Assess the outcome
Assess available thiols before coupling to a matched reagent such as maleimide.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
Traut's ReagentQH-70-7004
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untreated sample
Compare integrity and function with an untreated aliquot from the same preparation.
Reaction-pair control
Assess newly available thiols and changes with waiting time.
References
Thermo Fisher · Sulfhydryl-reactive chemistry
thermofisher.com
Explains thiol addition, blocking and disulfide exchange; maleimide, NEM, SPDP and Traut’s reagent have different purposes and products.
Frequently asked questions
Which handle reacts with Traut’s reagent first?
Traut’s reagent (2-iminothiolane) introduces thiols and is not a fluorophore. Newly installed thiols can oxidize, so assess them promptly and proceed to the next reaction.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
Assess available thiols before coupling to a matched reagent such as maleimide.