Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
SMCC
Cat. No.QH-70-7005
Connect primary-amine- and free-thiol-bearing components through NHS and maleimide ends.
Packs & quoteDefine the sequence
Validate at small scale
Separate and proceed
Product formNHS Ester/Maleimide
TargetPrimary amines and free thiols
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Reactive ends and coupling route
NHS couples to a primary amine and maleimide to a free thiol. Sequential activation and purification help control the process. Target disulfides are not free thiols.
Sequential bioconjugation
Connect primary-amine- and free-thiol-bearing components through NHS and maleimide ends.
Product and functional assessment
Assess component coupling, recovery, self-crosslinking and function rather than total fluorescence alone.
Specifications
Product information
- Product name
- SMCC
- Cat. No.
- QH-70-7005
- Product form
- NHS Ester/Maleimide
- Target
- Primary amines and free thiols
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 64987-85-5
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Plan the reaction sequence and remove excess linker and competing components such as DTT or β-mercaptoethanol.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Confirm the first target group and subsequent partner, then set input, pH, temperature and time for this reagent.
Validate at small scale
Plan the reaction sequence and remove excess linker and competing components such as DTT or β-mercaptoethanol.
Separate and proceed
Remove free reagents that interfere with the next step, retain the desired handle and complete the planned pairing.
Assess the outcome
Assess component coupling, recovery, self-crosslinking and function rather than total fluorescence alone.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
SMCCQH-70-7005
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untreated sample
Compare integrity and function with an untreated aliquot from the same preparation.
Reaction-pair control
Omit activation or a paired component to assess adsorption, residual reagents and nonspecific reaction.
References
Thermo Fisher · Amine-reactive chemistry
thermofisher.com
Distinguishes amine-reactive forms such as NHS and isothiocyanate and competing buffers; separately establish whether a nucleic-acid sample has accessible amines.
Thermo Fisher · Sulfhydryl-reactive chemistry
thermofisher.com
Explains thiol addition, blocking and disulfide exchange; maleimide, NEM, SPDP and Traut’s reagent have different purposes and products.
Stepwise amine-to-thiol coupling with SMCC
Thermo Fisher Scientific · SMCC and Sulfo-SMCC · MAN0011295 Rev. C.0
Distinguishes the partners and reaction order of the NHS and maleimide ends and removal of free linker, supporting bridging routes for NH2 dyes.
Reaction of Tris(2-carboxyethyl)phosphine (TCEP) with maleimide and alpha-haloacyl groups: anomalous elution of TCEP by gel filtration
Shafer DE, Inman JK, Lees A. · Analytical Biochemistry · 282(1):161–164 · 2000
Examines TCEP interactions with reactive groups, informing evaluation of reductant carryover and buffer exchange.
Frequently asked questions
How do this reagent’s handles participate in coupling?
NHS couples to a primary amine and maleimide to a free thiol. Sequential activation and purification help control the process. Target disulfides are not free thiols.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
Assess component coupling, recovery, self-crosslinking and function rather than total fluorescence alone.