Product manual
Product use, preparation, operating conditions and storage.
Linkers and crosslinkersCrosslinking reagent
1-Methylcyclopropene-PEG4-COOH
Cat. No.QH-70-7018
Activate the carboxyl to couple an amine-bearing molecule, retaining methylcyclopropene for a matched tetrazine reaction.
Packs & quoteDefine the sequence
Validate at small scale
Separate and proceed
Product formMethylcyclopropene/Free acid
TargetPrimary amines through activation; subsequently a matched tetrazine
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Reactive ends and coupling route
COOH is a free carboxylic acid requiring suitable activation. Methylcyclopropene can react with tetrazines, but substituents affect reactivity and stability; it is not a condition-matched substitute for TCO.
Sequential bioconjugation
Activate the carboxyl to couple an amine-bearing molecule, retaining methylcyclopropene for a matched tetrazine reaction.
Product and functional assessment
Assess time, product and background with the actual tetrazine partner.
Specifications
Product information
- Product name
- 1-Methylcyclopropene-PEG4-COOH
- Cat. No.
- QH-70-7018
- Product form
- Methylcyclopropene/Free acid
- Target
- Primary amines through activation; subsequently a matched tetrazine
- Pack sizes
- 50 mg / 100 mg / 250 mg
Preparation and properties
- CAS
- 2958710-53-5
- Storage
- Store at -20°C, protect from light and moisture
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Pre-reaction checks
Select carboxyl activation and purification that preserve the cyclopropene handle.
Solvent and molar calculation
Select solvent for this structure, salt form and solubility. Calculate molar inputs from its molecular weight; equal masses of different linkers are not equimolar.
Define the sequence
Confirm the first target group and subsequent partner, then set input, pH, temperature and time for this reagent.
Validate at small scale
Select carboxyl activation and purification that preserve the cyclopropene handle.
Separate and proceed
Remove free reagents that interfere with the next step, retain the desired handle and complete the planned pairing.
Assess the outcome
Assess time, product and background with the actual tetrazine partner.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
1-Methylcyclopropene-PEG4-COOHQH-70-7018
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untreated sample
Compare integrity and function with an untreated aliquot from the same preparation.
Reaction-pair control
Omit activation or a paired component to assess adsorption, residual reagents and nonspecific reaction.
References
Synthesis and reactivity comparisons of 1-methyl-3-substituted cyclopropene mini-tags for tetrazine bioorthogonal reactions
Yang J, Liang Y, Šečkutė J, Houk KN, Devaraj NK. · Chemistry – A European Journal · 20(12):3365–3375 · 2014
Compares substituent effects on methylcyclopropene reactivity and aqueous stability, supporting evaluation of the actual reaction pair.
Frequently asked questions
How do this reagent’s handles participate in coupling?
COOH is a free carboxylic acid requiring suitable activation. Methylcyclopropene can react with tetrazines, but substituents affect reactivity and stability; it is not a condition-matched substitute for TCO.
Does this reagent itself provide fluorescence?
It is used for coupling, modification, blocking or biotin deposition. Fluorescence requires a suitable fluorophore or detection system.
Can another linker’s conditions be reused?
Reactive ends, spacers, salt form and solubility can differ. Establish a small-scale reaction with this reagent and analyze the product.
How is the intended outcome confirmed?
Assess time, product and background with the actual tetrazine partner.