Record the target and formulation
Identify the molecule, available amount, reactive groups and complete storage-buffer composition.
AF fluorescent dyes
AF350–AF790 with NHS, Maleimide, Azide and DBCO derivatives, plus Annexin V, WGA, CTB and Streptavidin conjugates.
16 dyes80 Reactive dyes61 Bioconjugates
Product selectionProduct selection
Choose NHS ester for primary amines, maleimide for free thiols, and azide/DBCO for matching click handles; distinguish PEG4/PEG12 spacers. For ready-labeled probes, match the recognition target; Annexin V additionally requires a calcium-compatible system.
Showing 1–24 of 141 products
| Product / Cat. No. | Product form | Excitation / nm | Emission / nm | Pack sizes | Product details |
|---|---|---|---|---|---|
| AF350 NHS EsterQH-10-1001 | NHS Ester | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF350 MaleimideQH-10-1001(Maleimide) | Maleimide | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF350 AzideQH-10-1001(Azide) | Azide | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF350-PEG12-DBCOQH-10-1001(DBCO PEG12) | PEG12-DBCO | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF350-PEG4-DBCOQH-10-1001(DBCO PEG4) | PEG4-DBCO | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF350-StreptavidinQH-15-1001 | Streptavidin | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF350-Annexin VQH-10-1001(Annexin V) | Annexin V | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF350 WGAQH-10-1001(WGA) | WGA | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF350 CTBQH-10-1001(CTB) | CTB | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF405 NHS EsterQH-10-1002 | NHS Ester | 400 | 424 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF405 MaleimideQH-10-1002(Maleimide) | Maleimide | 400 | 424 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF405 AzideQH-10-1002(Azide) | Azide | 400 | 424 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF405-PEG4-DBCOQH-10-1002(DBCO PEG4) | PEG4-DBCO | 400 | 424 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF405-StreptavidinQH-15-1002 | Streptavidin | 400 | 424 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF405-Annexin VQH-10-1002(Annexin V) | Annexin V | 400 | 424 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF405 WGAQH-10-1002(WGA) | WGA | 400 | 424 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF405 CTBQH-10-1002(CTB) | CTB | 400 | 424 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF405-PEG12-DBCOQH-10-1002(DBCO PEG12) | PEG12-DBCO | 402 | 421 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF430 NHS EsterQH-10-1003 | NHS Ester | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF430 MaleimideQH-10-1003(Maleimide) | Maleimide | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF430 AzideQH-10-1003(Azide) | Azide | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF430-PEG12-DBCOQH-10-1003(DBCO PEG12) | PEG12-DBCO | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF430-PEG4-DBCOQH-10-1003(DBCO PEG4) | PEG4-DBCO | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF430-StreptavidinQH-15-1003 | Streptavidin | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF430-Annexin VQH-10-1003(Annexin V) | Annexin V | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF430 WGAQH-10-1003(WGA) | WGA | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF430 CTBQH-10-1003(CTB) | CTB | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF488 NHS EsterQH-10-1004 | NHS Ester | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF488 MaleimideQH-10-1004(Maleimide) | Maleimide | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF488 AzideQH-10-1004(Azide) | Azide | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF488-PEG12-DBCOQH-10-1004(DBCO PEG12) | PEG12-DBCO | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF488-PEG4-DBCOQH-10-1004(DBCO PEG4) | PEG4-DBCO | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF488-StreptavidinQH-15-1004 | Streptavidin | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF488-Annexin VQH-10-1004(Annexin V) | Annexin V | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF488 WGAQH-10-1004(WGA) | WGA | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF488 CTBQH-10-1004(CTB) | CTB | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF514 NHS EsterQH-10-1005 | NHS Ester | 517 | 542 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF514 MaleimideQH-10-1005(Maleimide) | Maleimide | 517 | 542 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF514 AzideQH-10-1005(Azide) | Azide | 517 | 542 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF514-PEG12-DBCOQH-10-1005(DBCO PEG12) | PEG12-DBCO | 517 | 542 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF514-PEG4-DBCOQH-10-1005(DBCO PEG4) | PEG4-DBCO | 517 | 542 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF514-Annexin VQH-10-1005(Annexin V) | Annexin V | 517 | 542 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF514 WGAQH-10-1005(WGA) | WGA | 517 | 542 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF514 CTBQH-10-1005(CTB) | CTB | 517 | 542 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF532 NHS EsterQH-10-1006 | NHS Ester | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF532 MaleimideQH-10-1006(Maleimide) | Maleimide | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF532 AzideQH-10-1006(Azide) | Azide | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF532-PEG12-DBCOQH-10-1006(DBCO PEG12) | PEG12-DBCO | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF532-PEG4-DBCOQH-10-1006(DBCO PEG4) | PEG4-DBCO | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF532-StreptavidinQH-15-1005 | Streptavidin | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF532-Annexin VQH-10-1006(Annexin V) | Annexin V | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF532 WGAQH-10-1006(WGA) | WGA | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF532 CTBQH-10-1006(CTB) | CTB | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF546 NHS EsterQH-10-1007 | NHS Ester | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF546 MaleimideQH-10-1007(Maleimide) | Maleimide | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF546 AzideQH-10-1007(Azide) | Azide | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF546-PEG12-DBCOQH-10-1007(DBCO PEG12) | PEG12-DBCO | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF546-PEG4-DBCOQH-10-1007(DBCO PEG4) | PEG4-DBCO | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF546-StreptavidinQH-15-1006 | Streptavidin | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF546-Annexin VQH-10-1007(Annexin V) | Annexin V | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF546 WGAQH-10-1007(WGA) | WGA | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF546 CTBQH-10-1007(CTB) | CTB | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF555 NHS EsterQH-10-1008 | NHS Ester | 555 | 572 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF555 MaleimideQH-10-1008(Maleimide) | Maleimide | 555 | 572 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF555 AzideQH-10-1008(Azide) | Azide | 555 | 572 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF555-PEG12-DBCOQH-10-1008(DBCO PEG12) | PEG12-DBCO | 555 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF555-PEG4-DBCOQH-10-1008(DBCO PEG4) | PEG4-DBCO | 555 | 572 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF555-StreptavidinQH-15-1007 | Streptavidin | 555 | 572 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF555-Annexin VQH-10-1008(Annexin V) | Annexin V | 555 | 572 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF555 WGAQH-10-1008(WGA) | WGA | 555 | 572 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF555 CTBQH-10-1008(CTB) | CTB | 555 | 572 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF568 NHS EsterQH-10-1009 | NHS Ester | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF568 MaleimideQH-10-1009(Maleimide) | Maleimide | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF568 AzideQH-10-1009(Azide) | Azide | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF568-PEG12-DBCOQH-10-1009(DBCO PEG12) | PEG12-DBCO | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF568-PEG4-DBCOQH-10-1009(DBCO PEG4) | PEG4-DBCO | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF568-StreptavidinQH-15-1008 | Streptavidin | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF568-Annexin VQH-10-1009(Annexin V) | Annexin V | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF568 WGAQH-10-1009(WGA) | WGA | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF568 CTBQH-10-1009(CTB) | CTB | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF594 NHS EsterQH-10-1010 | NHS Ester | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF594 MaleimideQH-10-1010(Maleimide) | Maleimide | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF594 AzideQH-10-1010(Azide) | Azide | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF594-PEG12-DBCOQH-10-1010(DBCO PEG12) | PEG12-DBCO | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF594-PEG4-DBCOQH-10-1010(DBCO PEG4) | PEG4-DBCO | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF594-StreptavidinQH-15-1009 | Streptavidin | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF594-Annexin VQH-10-1010(Annexin V) | Annexin V | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF594 WGAQH-10-1010(WGA) | WGA | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF594 CTBQH-10-1010(CTB) | CTB | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF647 NHS EsterQH-10-1011 | NHS Ester | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF647 MaleimideQH-10-1011(Maleimide) | Maleimide | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF647 AzideQH-10-1011(Azide) | Azide | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF647-PEG12-DBCOQH-10-1011(DBCO PEG12) | PEG12-DBCO | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF647-PEG4-DBCOQH-10-1011(DBCO PEG4) | PEG4-DBCO | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF647-StreptavidinQH-15-1010 | Streptavidin | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF647-Annexin VQH-10-1011(Annexin V) | Annexin V | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF647 WGAQH-10-1011(WGA) | WGA | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF647 CTBQH-10-1011(CTB) | CTB | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF660 NHS EsterQH-10-1012 | NHS Ester | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF660 MaleimideQH-10-1012(Maleimide) | Maleimide | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF660 AzideQH-10-1012(Azide) | Azide | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF660-PEG12-DBCOQH-10-1012(DBCO PEG12) | PEG12-DBCO | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF660-PEG4-DBCOQH-10-1012(DBCO PEG4) | PEG4-DBCO | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF660-StreptavidinQH-15-1011 | Streptavidin | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF660-Annexin VQH-10-1012(Annexin V) | Annexin V | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF660 WGAQH-10-1012(WGA) | WGA | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF660 CTBQH-10-1012(CTB) | CTB | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF680 NHS EsterQH-10-1013 | NHS Ester | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF680 MaleimideQH-10-1013(Maleimide) | Maleimide | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF680 AzideQH-10-1013(Azide) | Azide | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF680-PEG12-DBCOQH-10-1013(DBCO PEG12) | PEG12-DBCO | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF680-PEG4-DBCOQH-10-1013(DBCO PEG4) | PEG4-DBCO | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF680-StreptavidinQH-15-1012 | Streptavidin | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF680-Annexin VQH-10-1013(Annexin V) | Annexin V | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF680 WGAQH-10-1013(WGA) | WGA | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF680 CTBQH-10-1013(CTB) | CTB | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF700 NHS EsterQH-10-1014 | NHS Ester | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF700 MaleimideQH-10-1014(Maleimide) | Maleimide | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF700 AzideQH-10-1014(Azide) | Azide | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF700-PEG12-DBCOQH-10-1014(DBCO PEG12) | PEG12-DBCO | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF700-PEG4-DBCOQH-10-1014(DBCO PEG4) | PEG4-DBCO | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF700-StreptavidinQH-15-1013 | Streptavidin | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF700-Annexin VQH-10-1014(Annexin V) | Annexin V | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF700 WGAQH-10-1014(WGA) | WGA | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF700 CTBQH-10-1014(CTB) | CTB | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF750-PEG12-DBCOQH-10-1015(DBCO PEG12) | PEG12-DBCO | 749 | 775 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF750 NHS EsterQH-10-1015 | NHS Ester | 753 | 782 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF750 MaleimideQH-10-1015(Maleimide) | Maleimide | 753 | 782 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF750 AzideQH-10-1015(Azide) | Azide | 753 | 782 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF750-PEG4-DBCOQH-10-1015(DBCO PEG4) | PEG4-DBCO | 753 | 782 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF750-Annexin VQH-10-1015(Annexin V) | Annexin V | 753 | 782 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF750 WGAQH-10-1015(WGA) | WGA | 753 | 782 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF750 CTBQH-10-1015(CTB) | CTB | 753 | 782 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF790 NHS EsterQH-10-1016 | NHS Ester | 785 | 810 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF790 MaleimideQH-10-1016(Maleimide) | Maleimide | 785 | 810 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF790 AzideQH-10-1016(Azide) | Azide | 785 | 810 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF790-PEG12-DBCOQH-10-1016(DBCO PEG12) | PEG12-DBCO | 785 | 810 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF790-PEG4-DBCOQH-10-1016(DBCO PEG4) | PEG4-DBCO | 785 | 810 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF790-Annexin VQH-10-1016(Annexin V) | Annexin V | 785 | 810 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF790 WGAQH-10-1016(WGA) | WGA | 785 | 810 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| AF790 CTBQH-10-1016(CTB) | CTB | 785 | 810 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg |
AF spectra
Peak schematic · Not measuredNormalized intensity (schematic)
Wavelength / nm
All 16 dyes · Hover or tap a name for its peak
Reactive groups and labeling targets
Match the conjugation route to the available group on your target.
| Reactive form | Target group | Before labeling | Products |
|---|---|---|---|
NHS Ester | R–NH₂Primary amines | Check for competing amines such as Tris or glycine and carrier proteins such as BSA. | |
Maleimide | R–SHFree thiols | Check thiol accessibility, reduction conditions and residual reducing reagents. | |
Azide | C≡CTerminal / strained alkynes | Use CuAAC with terminal alkynes or SPAAC with strained alkynes; check sample compatibility. | |
DBCO | R–N₃Azides | Copper-free SPAAC with azides; compare PEG4 and PEG12 spacers for target accessibility. |
Fluorescent bioconjugates
Find a ready-made probe by ligand and binding target.
Compare primary-amine and thiol labeling of antibodies
NHS and Maleimide labeling guideFluorescent labeling workflow
Confirm the sample, reactive groups and detection conditions before labeling.
Choose the coupling chemistry
Match amines, thiols or click partners. For ready-made probes, search by biological ligand.
Check the source and collection window
Evaluate the excitation source, emission collection range and other dyes together.
Prepare using the specific product record
Confirm molecular weight, solvent, reaction conditions and purification requirements. Record the procedure and control results.
Example: amine labeling with AF405
AF405 labeling
Assess AF405 detection conditions, then review NHS sample preparation, labeling and purification.
Technical articles
NHS and maleimide labeling routes
NHS labels primary amines; maleimide labels free thiols. Additives and reduction requirements determine sample preparation.
Azide and DBCO click-reaction pairing
Terminal alkynes pair with azides through CuAAC; DBCO pairs with azides through copper-free SPAAC.
Detection and performance assessment of FITC, AF488 and SF488
Compare detection windows, labeling chemistry, signal intensity, photobleaching and pH response separately.
Interpretation of Annexin V positivity
Annexin V positivity indicates accessible PS. Interpret it with membrane integrity and other apoptosis markers.
Fixation and permeabilization conditions for WGA staining
Fixation and permeabilization can change glycan accessibility. Compare outlines, intracellular signal and background separately.
CTB signal and lipid raft interpretation
CTB fluorescence reports GM1-associated distribution; puncta alone do not establish native lipid-raft structure.
Effects of free biotin on detection
Free biotin occupies streptavidin-binding sites and can reduce detection of biotinylated targets.
References
References on reaction chemistry, ligand recognition and assay interpretation. Reagents and conditions belong to each study; these papers do not validate Qihe AF product performance or establish equivalence to Alexa Fluor products.
Labeling a protein with fluorophores using NHS ester derivitization
Nanda JS, Lorsch JR. · Methods in Enzymology · 536:87–94 · 2014
Uses FAM ester labeling of a protein to explain primary-amine chemistry and labeling-efficiency assessment. It informs NHS workflow design; input and reaction conditions depend on the actual dye and sample.
Fluorescent-labeled antibodies: Balancing functionality and degree of labeling
Vira S, Mekhedov E, Humphrey G, Blank PS. · Analytical Biochemistry · 402(2):146–150 · 2010
Uses FITC-labeled anti-HA antibody to assess changes in labeling density and antigen binding. It informs input-series design and post-purification functional testing; DOL alone is insufficient for selecting a conjugate.
A Strain-Promoted [3 + 2] Azide–Alkyne Cycloaddition for Covalent Modification of Biomolecules in Living Systems
Agard NJ, Prescher JA, Bertozzi CR. · Journal of the American Chemical Society · 126(46):15046–15047 · 2004
Introduces ring-strain-driven azide–alkyne coupling without copper, a foundational study in SPAAC bioconjugation.
DOI: 10.1021/ja044996f
A novel assay for apoptosis: flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V
Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. · Journal of Immunological Methods · 184(1):39–51 · 1995
Introduces fluorescence detection of exposed PS with Annexin V and informs interpretation alongside membrane integrity.
Labeling membrane glycoproteins or glycolipids with fluorescent wheat germ agglutinin
Chazotte B. · Cold Spring Harbor Protocols · 2011(5):pdb.prot5623 · 2011
Describes glycan-dependent membrane labeling with WGA, informing sample preparation and imaging design.
DOI: 10.1101/pdb.prot5623
Structured clustering of the glycosphingolipid GM1 is required for membrane curvature induced by cholera toxin
Kabbani AM, Raghunathan K, Lencer WI, Kenworthy AK, Kelly CV. · PNAS · 117(26):14978–14986 · 2020
Examines how multivalent CTB binding affects GM1 organization and membrane shape, highlighting probe-induced perturbation.
A monovalent streptavidin with a single femtomolar biotin binding site
Howarth M, Chinnapen DJF, Gerrow K, et al. · Nature Methods · 3:267–273 · 2006
Uses an engineered monovalent protein to investigate crosslinking by multivalent binding. It informs assessment of probe valency in membrane-protein motion and clustering studies.
DOI: 10.1038/nmeth861
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza A, Chang HD, Radbruch A, et al. · European Journal of Immunology · 51(12):2708–3145 · 2021
Provides methodological guidance on detection settings, controls and experimental records for downstream conjugate evaluation.
Request product documentation
Choose a document and enter your phone and email. Include the product lot number for a CoA.
Product manual
Product use, preparation, reaction conditions and storage.
Certificate of analysis (CoA)
Lot-specific test methods, results and acceptance criteria.
Safety data sheet (SDS)
Handling, storage and emergency response information.
AF dye selection FAQ
How do AF names, excitation peaks and laser lines differ?
The number in an AF name is not a measured peak or laser setting. Use the exact catalog number’s excitation/emission in the comparison table, then check source, emission window and other dyes. Distinguish excitation-light color from fluorescence emission.
Which sample handles do NHS, maleimide, azide and DBCO require?
NHS targets accessible primary amines; maleimide targets free thiols. Azide uses CuAAC with terminal alkynes or SPAAC with suitable strained alkynes; DBCO pairs with azides without copper. Cysteine does not necessarily provide a free thiol, and unmodified antibodies do not directly qualify for DBCO ligation.
Is a longer PEG4/PEG12 spacer always better?
Spacers affect handle accessibility and product behavior. Compare them for target structure, sample environment and function. Check each derivative’s molecular weight, solvent and storage by catalog number rather than inferring brightness, solubility or reaction performance from PEG count.
Is switching to PBS enough when an antibody contains Tris or BSA?
For NHS labeling, address competing amines such as Tris and glycine. BSA is another labelable protein and is not reliably separated from an antibody by ordinary dialysis or desalting. Use carrier-free material or a purification method that resolves the carrier.
Do AF-WGA, CTB and streptavidin need another conjugation step?
They are already fluorescent probes handled as aqueous protein formulations. WGA recognizes glycans, CTB supports GM1-related binding/trafficking, and streptavidin detects accessible biotin after free-biotin removal. Use recognition-specific background and localization controls.
Can AF-Annexin V use an ordinary calcium-free antibody-staining buffer?
Annexin V binding to phosphatidylserine requires calcium. Use a suitable binding buffer and check chelators such as EDTA. Interpret the result with a membrane-integrity dye and appropriate controls; Annexin V positivity alone does not establish the death stage.
Why does higher labeling density not always improve an AF conjugate?
Input ratio differs from purified labeling density. Overlabeling can impair function, and some dyes can quench. Remove free dye, calculate density with the corresponding parameters and assess binding, negative background and recovery at comparable input.