Nuclear staining, membrane integrity, nucleic-acid detection and covalent labeling need different reagents. Start with the experimental purpose, then compare sample compatibility, detection and formulation.
24 products
Distinguish nuclear staining, membrane integrity, nucleic-acid detection, covalent labeling and synthesis before comparing wavelengths and packs. Confirm preparation for each stock strength.
24 matching products
For support, include: Nuclear-staining, membrane-integrity or covalent-labeling purpose, sample state and detection channel
Hoechst 33342 supports live-cell nuclear staining. DAPI is commonly used in fixed specimens, with live-cell permeability validated separately. SYTO 9 binds DNA and RNA and is not restricted to nuclei.
Method background: Nuclear and nucleic-acid stains · Thermo Fisher ↗
These dyes primarily reach nucleic acids after membrane damage. Assess original viability before fixation or permeabilization changes the membrane barrier.
SYBR Green I primarily targets dsDNA; SYBR Gold supports dsDNA, ssDNA and RNA gel detection. Validate YOYO-1 and TOTO-3 nucleic-acid imaging for the actual sample.
Fluorophores such as FAM and ROX do not themselves provide nucleic-acid recognition. NHS ester, FITC and free-acid forms each need the appropriate reaction design.
Fluorescein and TAMRA phosphoramidites are oligonucleotide-synthesis reagents requiring the corresponding activation, coupling and deprotection conditions.
Distinguish free acids from NHS esters, plan carboxyl activation, amine coupling and product analysis, and retain positional-isomer identity for FAM and ROX.
Applies to: For free-acid FAM/ROX starting materials; a free acid is not a preactivated NHS ester.
These papers and manufacturer methods are selected for the current product use. Their reagents, conditions and parameters belong to the reported systems, not to measured Qihe product performance or universal operating conditions.
Thermo Fisher Scientific · Molecular Probes Handbook · Section 8.1
Distinguishes DNA preference, membrane permeability and suitable readouts among nucleic-acid stains.
Thermo Fisher Scientific · Molecular Probes Handbook · Section 15.2
Explains nucleic-acid dyes in membrane-integrity assays and considerations for combined controls.
Cossarizza A, Chang HD, Radbruch A, et al. · European Journal of Immunology · 51(12):2708–3145 · 2021
Provides methodological guidance on detection settings, controls and experimental records for downstream conjugate evaluation.
Thermo Fisher Scientific · SYBR Nucleic Acid Gel Stains · TD004
Selection of nucleic-acid type, gel staining and imaging conditions; reagent amounts depend on the actual formulation.
Thermo Fisher Scientific · Specialty Phosphoramidites
Explains dye-phosphoramidite incorporation during synthesis. Confirm coupling, cleavage and deprotection for the actual dye structure; this source does not establish a common process for TAMRA and fluorescein reagents.
Thermo Fisher Scientific · Carbodiimide crosslinker chemistry
Explains carboxyl activation, intermediate hydrolysis and self-crosslinking risks in multi-handle samples, informing free-acid dye activation and product analysis.
thermofisher.com
Distinguishes amine-reactive forms such as NHS and isothiocyanate and competing buffers; separately establish whether a nucleic-acid sample has accessible amines.
Select a catalog number and the information you need, then enter your phone and email for a reply. Include the vial lot number for a CoA.
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Product use, preparation, operating conditions and storage.
Spectra, measurement conditions and detection settings for the exact catalog number.
Lot-specific test results. Include the lot number from the vial.
Handling, storage and emergency response information.
Select by task. DAPI/Hoechst stain suitable samples; SYBR and YOYO/TOTO are binding dyes; reactive FAM/ROX forms covalently label matching handles; phosphoramidites belong in oligonucleotide synthesis. Sample requirements and processing differ.
Hoechst 33342 supports live-cell nuclear staining. DAPI is commonly used for fixed samples, with live-cell permeability validated separately. SYTO 9 binds both DNA and RNA, so its signal is not confined to nuclei. Nuclear positivity alone does not establish viability.
Fixation/permeabilization changes the membrane barrier, preventing inference of original viability. Assess integrity beforehand with known live/dead controls. Positivity alone does not distinguish apoptosis from other death mechanisms. PI DNA-content analysis separately needs RNA interference addressed.
These signals arise from nucleic-acid binding. SYBR Green I primarily targets dsDNA; SYBR Gold supports dsDNA, ssDNA and RNA gel detection. Control YOYO/TOTO dye:nucleic-acid ratios and validate downstream function. Binding-based staining is not covalent probe preparation.
The units express different information. Calculate dilution from the purchased formulation and intended use rather than matching volume alone. For gels, match nucleic-acid loading, standards and exposure and avoid saturation.
NHS ester and FITC require suitable amino modifications; free acids need a matching activation route. The 5-/6-isomer, reactive end and molecular weight are part of product identity. Select by catalog number and assess probe function after purification.
Phosphoramidites are synthesis-platform reagents requiring appropriate activation, coupling, cleavage and deprotection. Check the platform, solvent moisture control and dye tolerance, then verify full-length product and dye integrity after purification.
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