Product manual
Product use, preparation, operating conditions and storage.
Nucleic-acid stains and labeling reagentsDetection probe
SYTO-9(5mM)
Cat. No.QH-60-6003
A cell-permeant nucleic-acid stain for DNA and RNA in both live and dead cells.
Packs & quotePrepare the sample
Titrate this formulation
Acquire and interpret
Excitation485 nm
Emission498 nm
TargetAccessible DNA and RNA
Preparation and storage
- Preparation
- Soluble in water or DMSO
- Storage
- Store at -20°C, protect from light
Nucleic-acid binding and detection
SYTO 9 enters intact cells and binds DNA and RNA, while also staining damaged cells. Positivity alone does not demonstrate viability. Interpret nuclear, cytoplasmic or organelle signal in the sample context.
Cellular and bacterial nucleic-acid staining
Observe nucleic-acid-associated signal. Validate the paired dye, species and signal competition for dual-stain viability analysis.
Specifications
Product information
- Product name
- SYTO-9(5mM)
- Cat. No.
- QH-60-6003
- Product form
- Nucleic-acid stains and labeling reagents
- Target
- Accessible DNA and RNA
- Pack sizes
- 100 μl
Preparation and properties
- Excitation wavelength
- 485 nm
- Emission wavelength
- 498 nm
- Preparation and sample
- Soluble in water or DMSO
- Storage
- Store at -20°C, protect from light
Spectral information and detection
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Check formulation concentration
Confirm whether the pack is solid, an mM stock or a fold-concentrated solution. Do not reconstitute an existing solution as a solid or interchange molar and fold-concentration units.
Sample and channel
Define live or fixed preparation, check other dyes and channels, and record collection, staining and acquisition times.
Prepare the sample
Handle consistently and gently, retaining intact cells or applying suitable fixation for the objective. Do not pre-fix or permeabilize groups intended for original membrane-integrity assessment.
Titrate this formulation
Use the purchased formulation and instructions for concentration/time series, comparing background, positive separation and saturation.
Acquire and interpret
Set thresholds with unstained and suitable positive controls, matching acquisition settings. Include single-stain compensation or unmixing for multicolor assays.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
SYTO-9(5mM)QH-60-6003
Spectra and measurement conditions
Spectra, measurement conditions and detection settings for the exact catalog number.
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Unstained control
Establish sample autofluorescence and detection background.
Handling and dose controls
Compare doses and processing steps to assess changes caused by dye, sample loss or exposure.
References
Nucleic-acid stain binding and membrane permeability
Thermo Fisher Scientific · Molecular Probes Handbook · Section 8.1
Distinguishes DNA preference, membrane permeability and suitable readouts among nucleic-acid stains.
Frequently asked questions
Does a positive signal mean the cell is alive?
SYTO 9 stains both live and dead cells; positivity alone does not establish viability.
Why can SYTO 9 show cytoplasmic or organelle signal?
SYTO 9 binds DNA and RNA; interpret distribution using sample nucleic acids and permeability rather than treating every green signal as a nucleus.
Does a SYTO 9/PI green-to-red ratio directly give viability?
Dye pairing, species, cell condition and signal competition affect the result. Use known live/dead controls and validate thresholds and proportions in the actual system.