Product manual
Product use, preparation, operating conditions and storage.
Nucleic-acid stains and labeling reagentsDetection probe
Sybr Green I 10000X in DMSO
Cat. No.QH-60-6002
Preferential dsDNA binding for nucleic-acid gel staining and development of validated detection systems.
Packs & quoteLoad and separate
Titrate this formulation
Acquire and interpret
Excitation480 nm
Emission520 nm
TargetNucleic-acid samples, primarily dsDNA
Preparation and storage
- Preparation
- Soluble in water or DMSO
- Storage
- Store at -20°C, protect from light
Nucleic-acid band detection
Fluorescence from nucleic-acid-bound dye reveals bands. Nucleic-acid type, fragment length, staining and imaging influence brightness; match loading and exposure for comparisons.
Nucleic-acid gels
Begin with dsDNA gel staining; validate blanks and response range before extending to another detection system.
Specifications
Product information
- Product name
- Sybr Green I 10000X in DMSO
- Cat. No.
- QH-60-6002
- Product form
- Nucleic-acid stains and labeling reagents
- Target
- Nucleic-acid samples, primarily dsDNA
- Pack sizes
- 500 μl
Preparation and properties
- Excitation wavelength
- 480 nm
- Emission wavelength
- 520 nm
- CAS
- 163795-75-3
- Preparation and sample
- Soluble in water or DMSO
- Storage
- Store at -20°C, protect from light
Spectral information and detection
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Diluting the 10,000× concentrate
This product is a fold-concentrated solution; dilute for the corresponding gel workflow. A 10,000× designation is not 10,000 mM and does not use the molar-stock calculation for SYTO 9 or YOYO.
Sample and channel
Prepare a suitable gel, loading standard and imaging system. Check illumination, filters and exposure, avoiding image saturation.
Load and separate
Choose gel, loading and running conditions for fragment size and nucleic-acid type, including standards and blanks.
Titrate this formulation
Use the purchased formulation and instructions for concentration/time series, comparing background, positive separation and saturation.
Acquire and interpret
Compare bands at matched, unsaturated exposure. For quantification, establish a standard curve for the corresponding nucleic acid.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
Sybr Green I 10000X in DMSOQH-60-6002
Spectra and measurement conditions
Spectra, measurement conditions and detection settings for the exact catalog number.
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Blank and standards
Compare dye blank, nucleic-acid standards and samples to assess background and working range.
Handling and dose controls
Compare doses and processing steps to assess changes caused by dye, sample loss or exposure.
References
SYBR nucleic-acid gel staining methods
Thermo Fisher Scientific · SYBR Nucleic Acid Gel Stains · TD004
Selection of nucleic-acid type, gel staining and imaging conditions; reagent amounts depend on the actual formulation.
Frequently asked questions
Do SYBR Green I and SYBR Gold cover the same nucleic acids?
SYBR Green I primarily binds dsDNA. Do not automatically transfer SYBR Gold’s dsDNA, ssDNA and RNA gel-detection scope; validate additional uses separately.
Does a brighter band always mean more nucleic acid?
Brightness also depends on structure, staining and imaging. Use matched standards and unsaturated acquisition for quantification.
Does 10,000× establish a fixed molar concentration?
Fold concentration guides dilution for the intended working solution; it does not establish an unknown molecular weight or molar concentration. Follow this product’s gel workflow and match staining and exposure across samples.