Product manual
Product use, preparation, operating conditions and storage.
Nucleic-acid stains and labeling reagentsReactive dye
6-Fluorescein Phosphoramidite
Cat. No.QH-60-6018
Introduce a fluorophore during solid-phase oligonucleotide synthesis using compatible synthesis, deprotection and purification procedures.
Packs & quoteConfigure the synthesis cycle
Cleave and deprotect
Purify and identify the product
Excitation494 nm
Emission522 nm
TargetHydroxyl coupling sites in solid-phase oligonucleotide synthesis
Preparation and storage
- Storage
- Store at -20°C, protect from light and moisture
Labeling during solid-phase synthesis
A phosphoramidite introduces the fluorophore through activation and coupling within a synthesis cycle. It is not a reagent for directly labeling an already synthesized nucleic acid in water.
Fluorescent oligonucleotides
For fluorescent primers or probes with a workflow compatible with the sequence, incorporation position and deprotection conditions.
Specifications
Product information
- Product name
- 6-Fluorescein Phosphoramidite
- Cat. No.
- QH-60-6018
- Product form
- Phosphoramidite
- Target
- Hydroxyl coupling sites in solid-phase oligonucleotide synthesis
- Pack sizes
- 250 mg
Preparation and properties
- Excitation wavelength
- 494 nm
- Emission wavelength
- 522 nm
- CAS
- 204697-37-0
- Molar extinction coefficient
- 83,000 M⁻¹ cm⁻¹
- Quantum yield
- 0.93
- Storage
- Store at -20°C, protect from light and moisture
Spectral information and detection
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Anhydrous handling
Confirm the specified dry solvent, activator and synthesis concentration; moisture can compromise monomer activity.
Deprotection compatibility
Check the fluorophore’s tolerance to base, temperature and exposure time before selecting cleavage and deprotection.
Configure the synthesis cycle
Configure dissolution, activation, coupling and subsequent cycles for the synthesizer and reagent.
Cleave and deprotect
Use conditions compatible with this fluorophore and the oligonucleotide protecting groups.
Purify and identify the product
Use suitable HPLC, PAGE and mass spectrometry to assess full-length product, free dye and incompletely deprotected species.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
6-Fluorescein PhosphoramiditeQH-60-6018
Spectra and measurement conditions
Spectra, measurement conditions and detection settings for the exact catalog number.
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Synthesis control
Compare purity, sequence function and detection background of unlabeled and labeled oligonucleotides.
References
Fluorescent phosphoramidites in oligonucleotide synthesis
Thermo Fisher Scientific · Specialty Phosphoramidites
Explains dye-phosphoramidite incorporation during synthesis. Confirm coupling, cleavage and deprotection for the actual dye structure; this source does not establish a common process for TAMRA and fluorescein reagents.
Frequently asked questions
Can it be added directly to aqueous DNA?
It is intended for solid-phase synthesis cycles. Post-synthetic labeling requires a reagent matched to an existing handle.
Are deprotection conditions universal?
Tolerance differs among fluorophores; confirm conditions for this product.