Start with viability, DNA synthesis, nucleic-acid labeling or human-antibody internalization, then select a dye channel. Each workflow has its own sample and interpretation requirements.
23 products
Select the experimental endpoint before the channel. Viability, EdU, nucleic-acid labeling and antibody internalization each require their own preparation, sequence and controls.
23 matching products
For support, include: Experimental endpoint, cell or nucleic-acid sample, downstream fixation/permeabilization and detection channel
Cell-Life is an amine-reactive fixable viability dye. Compromised cells expose more intracellular protein sites and stain more strongly.
EdU incorporation occurs during DNA synthesis in living cells. This series uses copper-catalyzed azide–alkyne detection without a separate BrdU-style DNA-denaturation step.
Method background: Copper and fluorophore compatibility · Thermo Fisher ↗
Label IT does not require preintroduced amino or click handles. Control labeling through nucleic-acid quality, reagent input and incubation while retaining downstream function.
Confirm human-antibody suitability and labeling conditions in the dedicated instructions. Signal increases in acidic compartments and depends on uptake, local pH and antibody binding.
Review antibody suitability, reagent background and target-associated uptake with the anti-human labeling reagent.
Applies to: For QH-17-1003; direct TFP/maleimide covalent-labeling procedures do not apply.
These papers and manufacturer methods are selected for the current product use. Their reagents, conditions and parameters belong to the reported systems, not to measured Qihe product performance or universal operating conditions.
Perfetto SP, Chattopadhyay PK, Lamoreaux L, et al. · Journal of Immunological Methods · 313(1–2):199–208 · 2006
Examines titration, live/dead separation and post-fixation detection with amine-reactive viability dyes, informing control design for fixable staining.
Cossarizza A, Chang HD, Radbruch A, et al. · European Journal of Immunology · 51(12):2708–3145 · 2021
Provides methodological guidance on detection settings, controls and experimental records for downstream conjugate evaluation.
Salic A, Mitchison TJ. · PNAS · 105(7):2415–2420 · 2008
EdU incorporation is followed by fixation, permeabilization and fluorescent-azide CuAAC detection without BrdU-style DNA denaturation. EdU’s terminal alkyne is not a conventional copper-free SPAAC partner; the paper does not validate Qihe kit compatibility with GFP or PE.
Presolski SI, Hong VP, Finn MG. · Current Protocols in Chemical Biology · 3(4):153–162 · 2011
Describes CuAAC catalyst systems and reaction assessment for terminal-alkyne substrates.
Thermo Fisher Scientific · Click-iT EdU Flow Cytometry Assay Kits · MP10418, 2011
Explains the effects of copper-catalyzed EdU detection on GFP, PE and PE tandems and the importance of staining order. This is conventional Click-iT guidance; its formulation, doses and compatibility conclusions for other labels do not replace Qihe-kit validation or imply Plus-series compatibility.
Mirus Bio · Label IT Nucleic Acid Labeling Kit · ML002
Manufacturer background on direct chemical labeling of existing DNA/RNA, labeling density and purification recovery. Manufacturer catalog numbers, formulation and doses are not Qihe kit specifications; check recovery for short fragments.
Jin H, et al. · Oncoimmunology · 10(1):1958590 · 2021
Uses pHrodo-labeled avelumab and variants to assess Fcγ-receptor and PD-L1 contributions, informing background controls for anti-human screening. It does not study the binding mechanism or compatibility of Qihe QH-17-1003.
Select a catalog number and the information you need, then enter your phone and email for a reply. Include the vial lot number for a CoA.
Choose a product above to enable the request buttons.
Product use, preparation, operating conditions and storage.
Spectra, measurement conditions and detection settings for the exact catalog number.
Lot-specific test results. Include the lot number from the vial.
Handling, storage and emergency response information.
Compromised cells expose more intracellular protein sites and acquire stronger amine-reactive labeling. Fixation/permeabilization first changes that distinction. Stain and wash unfixed cells before downstream processing, using known live/dead references.
During staining these proteins consume amine-reactive dye, so use an instruction-supported BSA- and serum-free buffer. After staining and washing, introduce the buffers required by downstream antibody staining or fixation/permeabilization.
EdU incorporation occurs during DNA synthesis in living cells. This kit’s copper-catalyzed detection follows fixation, washing and permeabilization, without an additional BrdU-style DNA-denaturation step. Include a no-EdU control and choose the pulse for the study window.
Validate compatibility of the copper reaction with these signals and use a verified immunostaining order. Compatibility of another copper-protected formulation does not transfer to this kit. EdU positivity reports DNA synthesis during the pulse, not viability directly.
This kit chemically labels existing DNA/RNA without requiring those preintroduced handles. Use its dedicated buffer and addition order, compare input/time series, then remove free reagent and validate downstream function.
Length affects membrane retention and recovery. Validate the membrane or use purification suited to fragment length. After removing free label, assess integrity, recovery and hybridization, expression or delivery; total fluorescence does not replace those measures.
Confirm human-antibody suitability in the dedicated instructions and provide subclass, intact/fragment format and formulation. The anti-human name does not establish universal compatibility, and the reagent does not use a free TFP/NHS-dye labeling recipe.
Signal depends on binding, uptake and local pH. Interpret it with time courses, intracellular localization and acidification controls; fixation/permeabilization can also change the readout. Fluorescence changes alone neither quantify absolute uptake nor demonstrate degradation.
We use cookies and third-party analytics (Microsoft Clarity, Baidu Tongji, Google Analytics) to measure traffic and improve our service. Clarity includes session recording. You may decline — core site functionality is unaffected. View privacy policy
