Product manual
Product use, preparation, operating conditions and storage.
Label IT nucleic-acid labelingReagent kit
Label IT Cy3 Labeling Kit
Cat. No.QH-90-9001
Covalently attach a fluorophore to DNA or RNA through chemical labeling for nucleic-acid tracking and detection-method development.
Packs & quotePrepare a reference reaction
Label
Remove free reagent
Excitation550 nm
Emission570 nm
TargetPurified DNA or RNA
Preparation and storage
- Storage
- Store labeling reagent at −20°C, protected from light. Unopened components have a one-year shelf life under specified conditions; reconstituted reagent can be stored for six months. Store ultrafiltration components at room temperature as packaged.
Chemical nucleic-acid labeling
Label IT chemically labels nucleic acids covalently without requiring preintroduced amino or click handles. Extent of labeling depends on nucleic-acid quality, reagent input, salt and time; validate downstream hybridization, expression or transfection separately.
Nucleic-acid tracking
For fluorescence detection and localization of DNA or RNA after removing free reagent and checking nucleic-acid integrity.
Downstream functional studies
Assess hybridization, expression or delivery for the intended use rather than selecting labeling conditions from fluorescence alone.
Specifications
Product information
- Product name
- Label IT Cy3 Labeling Kit
- Cat. No.
- QH-90-9001
- Product form
- Label IT nucleic-acid labeling
- Target
- Purified DNA or RNA
- Pack sizes
- 25 μg / 100 μg
Preparation and properties
- Excitation wavelength
- 550 nm
- Emission wavelength
- 570 nm
- Molar extinction coefficient
- 150,000 M⁻¹ cm⁻¹
- Quantum yield
- 0.31
- Storage
- Store labeling reagent at −20°C, protected from light. Unopened components have a one-year shelf life under specified conditions; reconstituted reagent can be stored for six months. Store ultrafiltration components at room temperature as packaged.
- Reference reaction temperature
- 37°C
- Reference reaction time
- 1–3 hours
- Reaction buffer
- 1× final
Spectral information and detection
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Nucleic-acid quality and buffer
Use nuclease-free handling and check purity and salt. High salt can inhibit labeling; purify or exchange buffer if needed.
Reaction proportions
Use labeling buffer at 1× final concentration, keep labeling reagent below 20% of total volume and add it last.
Prepare a reference reaction
A reference 50 µL reaction for 5 µg nucleic acid contains 5 µL labeling reagent, 5 µL 10× buffer, 5 µL nucleic acid at 1 mg/mL and 35 µL nuclease-free water.
Label
The reference reaction is 1–3 hours at 37°C. Compare input and time for the nucleic-acid type and downstream function.
Remove free reagent
For suitable samples, use the accompanying 10 kDa ultrafiltration procedure with at least three washes. Validate retention and recovery for short fragments rather than relying on nominal cutoff alone. For absorbance quantification, choose ethanol precipitation or another validated cleanup under the accompanying procedure.
Assess and store
Assess recovery, nucleic-acid integrity and labeling density, then test function. Store labeled material at −20°C protected from light.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
Label IT Cy3 Labeling KitQH-90-9001
Spectra and measurement conditions
Spectra, measurement conditions and detection settings for the exact catalog number.
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Unlabeled nucleic acid
Compare integrity, expression, hybridization or delivery to assess effects of processing.
Free-reagent control
Confirm that post-purification signal follows the nucleic acid rather than free dye.
Input and time series
Select acceptable function and low free-dye background rather than maximum total fluorescence.
References
Label IT direct chemical labeling and purification of nucleic acids
Mirus Bio · Label IT Nucleic Acid Labeling Kit · ML002
Manufacturer background on direct chemical labeling of existing DNA/RNA, labeling density and purification recovery. Manufacturer catalog numbers, formulation and doses are not Qihe kit specifications; check recovery for short fragments.
Frequently asked questions
Is nucleic-acid function guaranteed after labeling?
Labeling density and processing can affect function. Validate hybridization, expression or transfection against an unlabeled control.
Can the crude reaction be used directly in cells?
Remove free reagent as specified. Its cellular distribution and background can differ from those of labeled nucleic acid.
Are Cy3, Cy5 and Fluorescein amounts interchangeable?
Use the corresponding kit conditions and signal requirements, matching nucleic-acid input and comparing downstream function.
Must DNA/RNA be modified with amines or azides first?
This series chemically labels existing DNA/RNA without requiring those handles. NHS-dye and click-dye reaction procedures do not apply.
Can short oligonucleotides use 10 kDa ultrafiltration directly?
Validate retention and recovery for the length, structure and purification method. Short fragments can be lost, so check a small sample first and assess fluorescence alongside integrity and function.