Technical Articles

Selection and application resources for fluorescent dyes, labeling reagents and labeling services

6 articles

pHrodo2026-10-22

Sample setup and controls for pH630 human-antibody labeling

Check the QH-17-1003 liquid formulation, antibody compatibility and background controls without applying a free-dye workflow.

pHrodo2026-10-22

Troubleshooting pHrodo background and weak signal

Localize the problem through cell-free response, binding and cellular readouts before increasing dye or antibody amounts.

pHrodo2026-10-22

Controls and readouts for pHrodo internalization assays

Separate target-mediated uptake, nonspecific uptake and acidification, and define what the fluorescence readout measures.

Fluorescence labeling · pHrodo2026-10-16

Antibody DOL calculation and evaluation

Learn how to calculate antibody degree of labeling from A280 and dye absorbance, apply the correct extinction coefficients, and troubleshoot free dye, incorrect corrections, and excessive labeling. Evaluate the result against performance in the intended assay.

Fluorescence labeling · pHrodo2026-10-15

NHS Ester and maleimide antibody labeling

NHS Esters label primary amines, while maleimides label free thiols. Choose a route based on available functional groups, formulation and reduction requirements, then verify the labeled antibody's function.

pHrodo2026-10-15

pHrodo selection for antibody internalization

Compare detection channels and labeling routes for pHAb, pH560 Red, pH630 Deep Red and the human-antibody companion reagent, with channel and sample considerations for internalization assays.