Distinguish peripheral and intracellular CTB signals over time; one endpoint cannot establish a trafficking route.
Dashed line: noncovalent target recognition. Shapes are schematic. AF marker: generic dye symbol.
State the start of the observation interval and retain acquisition times and display ranges. Data using different time origins should not be pooled into a single time course.
Describe peripheral and intracellular signals separately. When signal overlaps an organelle marker, exclude spillover and saturated pixels; spatial overlap alone does not establish a transport mechanism.
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