Product manual
Product use, preparation, operating conditions and storage.
Polyand transfection reagentsTransfection reagent
Polyand for Protein DNA Transfection Reagent
Cat. No.100006-2
For DNA transfection in recombinant protein and antibody expression, with the process set for the host cell and expression construct.
Packs & quoteDefine starting conditions
Prepare and add complexes
Culture and monitor viability
Product formTransfection reagent
TargetExpression plasmids and suitable hosts such as 293 or CHO cells
Preparation and storage
- Preparation
- See the product manual
- Storage
- Store at 2–8°C
DNA delivery and expression
Transfection complexes deliver expression DNA. Protein output depends on host, construct, cell state, culture and harvest; the transfected-cell fraction is not the target-protein yield.
Recombinant protein expression
For suitable 293 or CHO expression systems, comparing total expression, secreted product and purification recovery.
Antibody expression processes
Set DNA input and culture conditions for heavy/light-chain constructs and the host, assessing yield and functional quality.
Application data
Protein-expression example in CHO and 293 cells
Polyand for Protein DNA Transfection Reagent, Cat. No. 100006-2. The original brochure compares protein yield in CHO and 293 cells across complex incubation times; this is an application example, not a release specification.
- Cells: CHO and 293
- Readout: protein yield, mg/L
- The source does not specify the expression construct, DNA input, culture scale or harvest time.
Specifications
Product information
- Product name
- Polyand for Protein DNA Transfection Reagent
- Cat. No.
- 100006-2
- Product form
- Polyand transfection reagents
- Target
- Expression plasmids and suitable hosts such as 293 or CHO cells
- Pack sizes
- 1 ml
Preparation and properties
- Preparation and sample
- See the product manual
- Storage
- Store at 2–8°C
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Cells and DNA
Check cell state, density, viability and contamination; verify plasmid purity, integrity and component ratios.
Process conditions
Set DNA:reagent ratio, diluent, complexation time and culture conditions from this product’s instructions. Do not dissolve a transfection reagent in DMSO as if it were a reactive dye.
Define starting conditions
Use the procedure for the intended host and compare DNA and reagent inputs at small scale first.
Prepare and add complexes
Control addition order, complexation time and medium compatibility, matching cell density across groups.
Culture and monitor viability
Record cell state at matched time points and follow the planned feeding, medium-change or harvest procedure.
Analyze the final product
Measure target-protein yield, recovery and function, comparing matched harvest times and culture volumes.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
Polyand for Protein DNA Transfection Reagent100006-2
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Untransfected and reagent-only controls
Assess background and the effect of delivery treatment on viability.
Matched DNA and cells
Compare conditions with the same DNA batch and cells in a consistent state.
Yield and function
Assess expression, viability and product function together rather than comparing reporter signal alone.
Frequently asked questions
Are the two Polyand products interchangeable?
Virus and Protein target different outputs, DNA systems and harvest endpoints. Use the corresponding procedure.
Is there a universal ratio for every cell?
Cell type, DNA quality and culture process affect performance. Start with this product’s procedure and optimize at small scale.
How can storage and dosing details be obtained?
Request product instructions and provide cell type, culture mode, scale and intended output to confirm suitable conditions.