Product manual
Product use, preparation, operating conditions and storage.
BODIPY 564/570Bioconjugate
BODIPY 564/570 WGA
Cat. No.QH-40-4003(WGA)
Use fluorescent WGA to detect accessible glycans for cell-surface outlines and glycoconjugate localization.
Packs & quoteWGA
Fluorescent conjugateGlcNAc / Sia
GlcNAc / sialylated glycansBinding
Noncovalent recognitionExcitation565 nm
Emission574 nm
TargetAccessible N-acetylglucosamine- and sialic-acid-associated glycans
Preparation and storage
- Preparation
- Aqueous formulation
- Storage
- Store at 2–8°C, protect from light. Do not freeze
WGA glycan binding
WGA is wheat germ agglutinin. Signal depends on glycan distribution and accessibility. Fixation, permeabilization, temperature and incubation time affect the observed pattern; signal is not a measure of total membrane amount.
Surface outlines
Observe the surface with a suitable short staining period and compare localization with an independent membrane marker.
Glycan localization in fixed samples
Validate fixation and permeabilization separately to distinguish surface from accessible intracellular glycans.
Specifications
Product information
- Product name
- BODIPY 564/570 WGA
- Cat. No.
- QH-40-4003(WGA)
- Product form
- WGA
- Target
- Accessible N-acetylglucosamine- and sialic-acid-associated glycans
- Pack sizes
- 1 mg / 5 mg / 10 mg / 25 mg / 100 mg
Preparation and properties
- Excitation wavelength
- 565 nm
- Emission wavelength
- 574 nm
- Molar extinction coefficient
- 142,000 M⁻¹ cm⁻¹
- Preparation and sample
- Aqueous formulation
- Storage
- Store at 2–8°C, protect from light. Do not freeze
Spectral information and detection
Product parameters
Use parameters for the exact catalog number when preparing reagents, setting reagent input or calculating results. Request a lot-specific CoA for test results.
Protocol
Define sample processing
Record cell type, fixation, permeabilization and buffer. Titrate live and fixed samples separately.
Handle the protein conjugate
Dilute according to this formulation. Do not prepare a WGA protein conjugate using organic-solvent instructions for a reactive free dye.
Titrate the probe
Compare probe amount and incubation time at matched cell counts, retaining an unstained sample.
Control time and temperature
Plan incubation, washes and chase periods separately for surface staining and uptake studies, matching handling before acquisition.
Wash and image
Remove unbound probe using a suitable wash procedure and compare background and localization with matched exposure or instrument settings.
Request product documentation
Select a document and enter your phone and email. Include the lot number for a CoA.
BODIPY 564/570 WGAQH-40-4003(WGA)
Spectra and measurement conditions
Spectra, measurement conditions and detection settings for the exact catalog number.
Certificate of analysis (CoA)
Lot-specific test results. Include the lot number from the vial.
Safety data sheet (SDS)
Handling, storage and emergency response information.
Quality and lot information
Unstained and single-stain controls
Establish autofluorescence, channel spillover and thresholds.
Processing controls
Compare fixation, permeabilization and incubation conditions in pairs to distinguish accessibility changes from expression changes.
Localization control
Use independent surface or organelle markers to assess surface binding and intracellular signal.
Technical articles
WGA signal and membrane outlines
WGA labels accessible glycans. Check outline continuity for segmentation; intensity is not a direct measure of membrane amount.
For WGA conjugates; glycan accessibility and membrane outlines do not establish protein-specific targeting.
Fixation and permeabilization conditions for WGA staining
Fixation and permeabilization can change glycan accessibility. Compare outlines, intracellular signal and background separately.
For comparing fixation and permeabilization with WGA probes, not for free-dye conjugation.
Application support
Include your sample, formulation and detection method when requesting application guidance.
References
Labeling membrane glycoproteins or glycolipids with fluorescent wheat germ agglutinin
Chazotte B. · Cold Spring Harbor Protocols · 2011(5):pdb.prot5623 · 2011
Describes glycan-dependent membrane labeling with WGA, informing sample preparation and imaging design.
DOI: 10.1101/pdb.prot5623
Frequently asked questions
Does WGA stain only the plasma membrane?
WGA recognizes glycans. Uptake, fixation and permeabilization can increase intracellular labeling; interpret localization for the sample conditions.
Should it be conjugated like a free dye?
It is already a fluorescent WGA conjugate for affinity staining, not a free dye awaiting covalent coupling to the sample.
Is there a universal staining amount?
Titrate for cell count, processing and lot concentration, selecting clear outlines with low extracellular background.