Choose a tandem by donor system and acceptor emission. Account for lot, fixation and single-color controls in multicolor assays; peak positions do not replace a full spectral reference.
8 products
Match donor excitation and acceptor emission and confirm the tandem material’s activation state. Establish compensation or unmixing with the actual conjugate, matching lot and processing, and monitor donor leakage.
8 matching products
| Product / Cat. No. | Excitation / nm | Emission / nm | Pack sizes | Product details |
|---|---|---|---|---|
| Percp-Cy5.5QH-50-5008 | 489 | 679 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| PE-Texas RedQH-50-5012 | 565 | 600 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| RPE-Cy5QH-50-5006 | 565 | 667 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| RPE-Cy5.5QH-50-5005 | 565 | 694 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| RPE-Cy7QH-50-5007 | 565 | 785 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| APC-AF700QH-50-5009 | 651 | 710 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| APC-AF750QH-50-5010 | 651 | 780 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| APC-Cy7QH-50-5011 | 651 | 780 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg |
For support, include: Donor system, instrument channels, fixation/permeabilization and intended lot
This page lists tandem fluorescent materials. The RPE and APC tandem manuals provide SMCC-mediated antibody-coupling examples; follow separate instructions for preactivated material and other donor products.
The donor determines the main excitation route and the acceptor the main output band. Nearby emission peaks do not make spectral references interchangeable between tandems.
Method background: Tandem dye controls · R&D Systems ↗
These papers and manufacturer methods are selected for the current product use. Their reagents, conditions and parameters belong to the reported systems, not to measured Qihe product performance or universal operating conditions.
R&D Systems · Tandem Dye-Conjugated Antibodies FAQs
The source concerns conjugated antibodies and informs energy transfer, degradation and lot-matched controls. Qihe’s tandem series contains labeling reagents; validate the final conjugate with lot- and process-matched single stains. The source is not a raw-reagent conjugation recipe.
Cossarizza A, Chang HD, Radbruch A, et al. · European Journal of Immunology · 51(12):2708–3145 · 2021
Provides methodological guidance on detection settings, controls and experimental records for downstream conjugate evaluation.
Select a catalog number and the information you need, then enter your phone and email for a reply. Include the vial lot number for a CoA.
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Product use, preparation, operating conditions and storage.
Spectra, measurement conditions and detection settings for the exact catalog number.
Lot-specific test results. Include the lot number from the vial.
Handling, storage and emergency response information.
The donor determines the main excitation route and the acceptor the main output band. Check donor, laser and collection window together. A shared Cy7 acceptor does not make excitation conditions or spectral references interchangeable.
These products are tandem fluorescent materials. RPE/APC instructions give SMCC-mediated examples: prepare material maleimide and antibody thiols separately, remove residual linker and reductant, then couple. Handle preactivated material according to its supplied reactive group.
A PE/APC example does not establish conditions for PerCP. Confirm PerCP material activation, molecular weight and formulation, then use its own coupling and purification information. The final conjugate still needs a matching single-stain reference.
Tandem spectra can depend on lot and processing. Use the actual conjugate with matching lot and fixation/permeabilization for compensation or unmixing, and recheck after changes rather than reusing a reference by dye name alone.
Check whether light, fixation/permeabilization or storage has altered the tandem, comparing matching single stains and fresh samples. Acceptor loss with increased donor leakage does not directly establish altered target expression. Store material and conjugate according to their own formulations.
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