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Cellular Component Labeling

Qihe Biotech offers fluorescent labeling reagents covering all major subcellular structures — including the nucleus, mitochondria, plasma membrane, lysosomes and endoplasmic reticulum — to support cell-biology imaging research.

Nucleus

Fluorescent labeling of DNA/RNA; commonly used nucleic acid dyes include DAPI, Hoechst and SYTOX.

Ex 350–500 nm
DAPIHoechst 33342SYTOX GreenPI (Propidium Iodide)7-AAD

Mitochondria

Membrane-potential-dependent dyes that accumulate in the mitochondrial matrix proportional to ΔΨm.

Ex 488–580 nm
MitoTracker GreenMitoTracker Red CMXRosJC-1TMRMRhodamine 123

Plasma Membrane

Lipophilic dyes intercalate into the lipid bilayer, or lectins/antibodies label membrane proteins.

Ex 484–644 nm
DiI/DiO/DiDCellMask Deep RedWGA LectinFM 1-43PKH series

Lysosome

Weak base dyes become protonated and trapped in acidic lysosomes (pH 4.5–5.0).

Ex 465–577 nm
LysoTracker RedLysoTracker GreenLysoSensor Yellow/BlueDAMP

Endoplasmic Reticulum

ER-Tracker series selectively binds sulfonylurea receptors on the endoplasmic reticulum.

Ex 484–587 nm
ER-Tracker RedER-Tracker GreenDiOC6(3)

Golgi Apparatus

Ceramide analogs (BODIPY FL C5-Ceramide) or lectins label Golgi membranes.

Ex 466–503 nm
BODIPY FL CeramideNBD C6-CeramideWGAGolgi-RFP

Cytoskeleton

Phalloidin selectively binds F-actin; taxol analogs label microtubules.

Ex 488–650 nm
Phalloidin (fluorescent conjugate)SiR-ActinTubulin TrackerPaclitaxel fluorescent conjugate

Lipid Droplets

Neutral lipid dyes selectively accumulate in the hydrophobic core of lipid droplets.

Ex 488–552 nm
BODIPY 493/503Nile RedLipidTOXHCS LipidTOX

Multi-Color Labeling Strategy

Recommended four-color live-cell imaging panel:

Nucleus
Hoechst 33342
Ex 350 / Em 461
Mitochondria
MitoTracker Green
Ex 490 / Em 516
Lysosome
LysoTracker Red
Ex 577 / Em 590
Plasma Membrane
CellMask Deep Red
Ex 649 / Em 666

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