Flow Cytometry Reagents
Qihe Biotech supplies 75 in-stock reagents for flow cytometry, covering viability, apoptosis, proliferation, DNA content analysis and fluorescent labeling.
Qihe Biotech does not sell pre-conjugated flow cytometry antibodies. To label your own antibody, use a reactive dye in NHS ester or maleimide form; for a biotinylated antibody, use a streptavidin conjugate as the secondary detection step.
Excitation maxima of these reagents span 330 nm to 787 nm, across the range of the common 355 / 405 / 488 / 561 / 640 nm laser lines. Each product page lists that product's own excitation/emission maxima; the usable channel depends on your instrument lasers and filters.
Viability dyes14 products
The Cell-Life Dye series stains dead cells specifically, so they can be excluded before analysis; the series covers a range of excitation/emission wavelengths.
Apoptosis · Annexin V conjugates16 products
Annexin V binds phosphatidylserine externalized early in apoptosis, and is commonly paired with a nucleic acid stain to separate apoptosis from necrosis. The same probe is available in several fluorescent channels.
Fluorescent proteins and tandem dyes12 products
R-PE, B-PE, APC and PerCP fluorescent proteins, plus RPE-Cy7, APC-Cy7 and Percp-Cy5.5 tandem dyes — the fluorophores most used in multicolor flow panels.
Nucleic acid and DNA content stains5 products
PI, 7-AAD, DAPI, Hoechst 33342 and SYTOX Green, for viability calls and cell cycle / DNA content analysis.
Proliferation · EdU kits4 products
EdU cell proliferation assay kits detect individual proliferating cells and quantify proliferation across a sample; the product documentation lists flow cytometry among the supported readouts.
Streptavidin conjugates24 products
Secondary detection for biotinylated antibodies: one biotinylated antibody moves to a different fluorescent channel simply by changing the streptavidin conjugate.
Frequently asked questions
Does Qihe Biotech supply flow cytometry antibodies?
No. Qihe Biotech supplies fluorescent dyes and detection reagents: label your own antibody with a reactive dye in NHS ester or maleimide form, or detect a biotinylated antibody with a streptavidin conjugate.
Which laser lines do these reagents cover?
Excitation maxima of the in-stock flow reagents span 330 nm to 787 nm, across the range of the common 355 / 405 / 488 / 561 / 640 nm laser lines. Each product page lists its own excitation/emission maxima.
How do I get the certificate of analysis for a specific lot?
Every Qihe Biotech production lot is confirmed by both NMR and HPLC-MS. Retrieve the certificate for a given lot on the CoA lookup page using the product number and lot number.