Prepare fluorescent conjugates for flow cytometry and imaging. Choose the detection band, then match an NH2 or DBCO route to your sample.
32 products
Choose NH2 for a sequential linker-mediated route, or DBCO for an azide-bearing sample. Match the detection band and compare the exact product parameters below.
Showing 1–24 of 32 products
| Product / Cat. No. | Product form | Excitation / nm | Emission / nm | Pack sizes | Product details |
|---|---|---|---|---|---|
| SF350-NH2QH-12-1001(NH2) | NH2 | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF350-DBCOQH-12-1001(DBCO) | DBCO | 346 | 445 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF405-NH2QH-12-1002(NH2) | NH2 | 400 | 424 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF405-DBCOQH-12-1002(DBCO) | DBCO | 400 | 424 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF435-NH2QH-12-1003(NH2) | NH2 | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF435-DBCOQH-12-1003(DBCO) | DBCO | 430 | 545 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF488-NH2QH-12-1004(NH2) | NH2 | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF488-DBCOQH-12-1004(DBCO) | DBCO | 494 | 517 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF514-NH2QH-12-1005(NH2) | NH2 | 517 | 542 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF514-DBCOQH-12-1005(DBCO) | DBCO | 517 | 542 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF532-NH2QH-12-1006(NH2) | NH2 | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF532-DBCOQH-12-1006(DBCO) | DBCO | 530 | 555 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF546-NH2QH-12-1007(NH2) | NH2 | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF546-DBCOQH-12-1007(DBCO) | DBCO | 554 | 570 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF555-NH2QH-12-1008(NH2) | NH2 | 555 | 572 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF555-DBCOQH-12-1008(DBCO) | DBCO | 555 | 572 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF568-NH2QH-12-1009(NH2) | NH2 | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF568-DBCOQH-12-1009(DBCO) | DBCO | 578 | 602 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF594-NH2QH-12-1010(NH2) | NH2 | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF594-DBCOQH-12-1010(DBCO) | DBCO | 590 | 617 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF647-NH2QH-12-1011(NH2) | NH2 | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF647-DBCOQH-12-1011(DBCO) | DBCO | 651 | 672 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF660-NH2QH-12-1012(NH2) | NH2 | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF660-DBCOQH-12-1012(DBCO) | DBCO | 668 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF680-NH2QH-12-1013(NH2) | NH2 | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF680-DBCOQH-12-1013(DBCO) | DBCO | 684 | 707 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF700-NH2QH-12-1014(NH2) | NH2 | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF700-DBCOQH-12-1014(DBCO) | DBCO | 702 | 723 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF750-NH2QH-12-1015(NH2) | NH2 | 753 | 782 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF750-DBCOQH-12-1015(DBCO) | DBCO | 753 | 782 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF790-NH2QH-12-1016(NH2) | NH2 | 785 | 810 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| SF790-DBCOQH-12-1016(DBCO) | DBCO | 785 | 810 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg |
Need help with your sample, chemistry or detection setup?
Original Mouse CD4 (GK1.5) flow cytometry data for SF488 and SF647. Compare the stated conditions and readout before applying the result to another conjugate.
SF488 and Qihe AF488, Mouse CD4 (GK1.5), 0.4 µg/test. CD4+ gated events: 23.08% and 20.59%. Gated percentages alone do not measure intrinsic dye brightness.
SF647 and Qihe AF647, Mouse CD4 (GK1.5), 0.08 µg/test. CD4+ gated events: 24.42% and 19.68%. Gated percentages alone do not measure intrinsic dye brightness.
A free amine on the dye; prepare the reactive intermediate before coupling to the target.
The SF manual describes an aqueous PBS preparation for this route. NH2 is not an NHS ester and does not directly couple two amines.
For a target carrying accessible azides; use copper-free SPAAC coupling.
Choose buffers compatible with the sample and click reaction. The NH2/SMCC procedure does not apply to DBCO.
Compare detection windows, labeling chemistry, signal intensity, photobleaching and pH response separately.
Applies to: Includes a defined Qihe SF488/AF488 flow example; it does not rank brightness or photostability against original Alexa Fluor 488.
Couple the dye amine, isolate the intermediate and react it with target thiols; check free SMCC, competing reductants and small-molecule separation.
Applies to: For NH2 dyes converted to maleimides via SMCC before thiol coupling; validate separation of the small-molecule intermediate.
Target-bound azides enable coupling; free azide reagents and sodium azide can compete for DBCO.
Applies to: For the SPAAC step between DBCO and a target azide; ordinary amines and thiols are not reaction partners.
These papers and manufacturer methods are selected for the current product use. Their reagents, conditions and parameters belong to the reported systems, not to measured Qihe product performance or universal operating conditions.
Thermo Fisher Scientific · SMCC and Sulfo-SMCC · MAN0011295 Rev. C.0
Distinguishes the partners and reaction order of the NHS and maleimide ends and removal of free linker, supporting bridging routes for NH2 dyes.
Shafer DE, Inman JK, Lees A. · Analytical Biochemistry · 282(1):161–164 · 2000
Examines TCEP interactions with reactive groups, informing evaluation of reductant carryover and buffer exchange.
Debets MF, van Berkel SS, Schoffelen S, et al. · Chemical Communications · 46(1):97–99 · 2010
Reports copper-free reaction of a strained alkyne with azides and its application to enzyme PEGylation.
DOI: 10.1039/b917797c
Agard NJ, Prescher JA, Bertozzi CR. · Journal of the American Chemical Society · 126(46):15046–15047 · 2004
Introduces ring-strain-driven azide–alkyne coupling without copper, a foundational study in SPAAC bioconjugation.
DOI: 10.1021/ja044996f
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Product use, preparation, operating conditions and storage.
Spectra, measurement conditions and detection settings for the exact catalog number.
Lot-specific test results. Include the lot number from the vial.
Handling, storage and emergency response information.
SF-NH2 carries a free amine. The manual’s SMCC route first couples that amine to the linker’s NHS end, removes free linker, then couples to target thiols. Two free amines do not couple directly.
First confirm accessible azide handles. An antibody’s amines do not provide the azide partner required for DBCO SPAAC. Remove free azide-installation reagent, then confirm buffer and solvent for the exact product and sample.
The SF manual provides an aqueous PBS preparation for the NH2 bridging route. Confirm DBCO stock preparation, input and sample compatibility separately; its workflow does not inherit the NH2 preparation or SMCC step.
The page shows specific Mouse CD4 (GK1.5) flow-cytometry examples without identifying an NH2 or DBCO catalog number. Evaluate other colors, forms and antibodies using the actual conjugate’s positive separation, negative background and binding.
Input ratio differs from purified labeling density. Remove free dye, use the corresponding optical parameters, then assess binding, background and recovery at comparable antibody input. Total reaction-mixture fluorescence is insufficient.
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