Compare neighboring models individually. Match the excitation and emission of each catalog number to the detection window, then confirm amine-labeling conditions.
7 products
QF405A/B differ in emission; QF415/500 have nearby emission but different excitation peaks. Match the exact catalog number to the channel. These are NHS esters; other QF-MAL or DBCO versions require their own instructions.
7 matching products
| Product / Cat. No. | Excitation / nm | Emission / nm | Pack sizes | Product details |
|---|---|---|---|---|
| QF405A NHS EsterQH-11-QF405A | 400 | 420 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| QF405B NHS EsterQH-11-QF405B | 408 | 452 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| QF415 NHS EsterQH-11-QF415 | 410 | 501 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| QF500 NHS EsterQH-11-QF500 | 426 | 498 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| QF488 NHS EsterQH-11-QF488 | 491 | 516 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| QF594 NHS EsterQH-11-QF594 | 593 | 614 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| QF700 NHS EsterQH-11-QF700 | 681 | 698 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg |
For support, include: Selected QF model, light source and filters, target sample and formulation
QF405A and QF405B differ in emission. QF415 and QF500 have nearby emission peaks but different excitation peaks. Model numbers alone do not specify laser settings or multicolor separation.
Establish accessible primary amines, sample formulation and readout conditions, then obtain preparation, input and calculation parameters for the selected NHS catalog number.
Tris and glycine consume NHS dye. BSA and gelatin require separation from the target protein; routine buffer exchange is insufficient.
Applies to: For protein preparation before NHS-ester labeling; check competing amines and carrier proteins.
Calculate DOL with absorbance correction, distinguish input ratio from attached dye, and assess recovery and binding.
Applies to: For purified protein conjugates; use this dye’s absorbance parameters and distinguish input ratio from DOL.
These papers and manufacturer methods are selected for the current product use. Their reagents, conditions and parameters belong to the reported systems, not to measured Qihe product performance or universal operating conditions.
Nanda JS, Lorsch JR. · Methods in Enzymology · 536:87–94 · 2014
Uses FAM ester labeling of a protein to explain primary-amine chemistry and labeling-efficiency assessment. It informs NHS workflow design; input and reaction conditions depend on the actual dye and sample.
Vira S, Mekhedov E, Humphrey G, Blank PS. · Analytical Biochemistry · 402(2):146–150 · 2010
Uses FITC-labeled anti-HA antibody to assess changes in labeling density and antigen binding. It informs input-series design and post-purification functional testing; DOL alone is insufficient for selecting a conjugate.
Select a catalog number and the information you need, then enter your phone and email for a reply. Include the vial lot number for a CoA.
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Product use, preparation, operating conditions and storage.
Spectra, measurement conditions and detection settings for the exact catalog number.
Lot-specific test results. Include the lot number from the vial.
Handling, storage and emergency response information.
They have different emission peaks. Even when used in a similar excitation region, check emission windows, sample background and other dyes separately. The shared 405 label does not make all acquisition settings interchangeable; use the product comparison values.
Their emission positions are close but excitation peaks differ. Check both source and collection window when switching. Validate spillover with actual single stains before using them as a two-color pair; different model numbers do not establish separation.
This page lists NHS ester versions for primary amines, while PEG12-MAL targets free thiols. Spacer, reaction partner and preparation differ; use the preparation and conditions for the selected NHS catalog number.
Remove free dye and record each conjugate’s labeling density and recovery. Use the same target sample and comparable antibody input with negative, single-stain and binding controls. Raw intensities from different channels or acquisition gains do not directly rank performance.
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