Label proteins, peptides and amino-modified molecules through accessible primary amines. Compare exact product wavelengths and match them to your instrument.
10 products
These products are NHS esters. ATTO 590/594 and ATTO 633/647 have nearby emission bands; compare actual excitation, filters and single-stain spillover before selecting channels.
10 matching products
| Product / Cat. No. | Excitation / nm | Emission / nm | Pack sizes | Product details |
|---|---|---|---|---|
| ATTO 390 NHS EsterQH-20-2001 | 390 | 479 | 1 mg | |
| ATTO 425 NHS EsterQH-20-2002 | 436 | 484 | 1 mg | |
| ATTO 488 NHS EsterQH-20-2003 | 453 | 508 | 1 mg | |
| ATTO 532 NHS EsterQH-20-2004 | 532 | 553 | 1 mg | |
| ATTO 550 NHS EsterQH-20-2005 | 554 | 576 | 1 mg | |
| ATTO 590 NHS EsterQH-20-2006 | 594 | 624 | 1 mg | |
| ATTO 594 NHS EsterQH-20-2007 | 601 | 627 | 1 mg | |
| ATTO 633 NHS EsterQH-20-2008 | 629 | 657 | 1 mg | |
| ATTO 647 NHS EsterQH-20-2009 | 645 | 669 | 1 mg | |
| ATTO 680 NHS EsterQH-20-2010 | 680 | 700 | 1 mg |
Need help with your sample, chemistry or detection setup?
ATTO 590 and 594 have nearby emission peaks, as do ATTO 633 and 647. Compare the exact product wavelengths, light source and emission passband; neighboring model numbers do not establish independent channels.
Tris and glycine consume NHS dye. BSA and gelatin require separation from the target protein; routine buffer exchange is insufficient.
Applies to: For protein preparation before NHS-ester labeling; check competing amines and carrier proteins.
Calculate DOL with absorbance correction, distinguish input ratio from attached dye, and assess recovery and binding.
Applies to: For purified protein conjugates; use this dye’s absorbance parameters and distinguish input ratio from DOL.
These papers and manufacturer methods are selected for the current product use. Their reagents, conditions and parameters belong to the reported systems, not to measured Qihe product performance or universal operating conditions.
Nanda JS, Lorsch JR. · Methods in Enzymology · 536:87–94 · 2014
Uses FAM ester labeling of a protein to explain primary-amine chemistry and labeling-efficiency assessment. It informs NHS workflow design; input and reaction conditions depend on the actual dye and sample.
Vira S, Mekhedov E, Humphrey G, Blank PS. · Analytical Biochemistry · 402(2):146–150 · 2010
Uses FITC-labeled anti-HA antibody to assess changes in labeling density and antigen binding. It informs input-series design and post-purification functional testing; DOL alone is insufficient for selecting a conjugate.
ATTO-TEC / Leica Microsystems · ATTO Antibody Labeling Kit · 2025-03-19
Manufacturer IgG/NHS-ester example for preparation, purification and absorbance measurements. Its catalog numbers, spectral peaks, extinction coefficients and kit doses do not replace documentation for Qihe catalog numbers.
Select a catalog number and the information you need, then enter your phone and email for a reply. Include the vial lot number for a CoA.
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Product use, preparation, operating conditions and storage.
Spectra, measurement conditions and detection settings for the exact catalog number.
Lot-specific test results. Include the lot number from the vial.
Handling, storage and emergency response information.
These models have neighboring emission bands. Model numbers do not specify laser lines or independent channels. Compare exact product excitation/emission with instrument passbands, then assess spillover and compensation or unmixing using actual single-stained conjugates.
Use the molecular weight of this catalog number’s NHS derivative for input calculations and its corresponding extinction and absorbance corrections for labeling density. Free-acid, other reactive-form or other-supplier data do not replace these values.
These products are NHS esters whose standard route requires suitable accessible primary amines. For nucleic acids, confirm an appropriate introduced amino handle; nitrogen atoms in native bases do not by themselves establish suitability for that route.
Remove competing amines such as Tris by a suitable buffer exchange. BSA is another labelable protein and is not reliably separated from an antibody by simple desalting. Prefer carrier-free material or establish a purification method that resolves the carrier.
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