Compare R-PE, B-PE, APC and PerCP for fluorescent conjugate development. Confirm the supplied formulation and coupling route, then assess signal and function after purification.
4 products
R-PE, B-PE, APC and PerCP are conjugation materials. Select by exact peaks, formulation and activation state. Attach a targeting molecule and plan purification that resolves free fluorescent protein from conjugate.
4 matching products
| Product / Cat. No. | Excitation / nm | Emission / nm | Pack sizes | Product details |
|---|---|---|---|---|
| PercpQH-50-5004 | 488 | 675 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| B-PEQH-50-5002 | 545 | 578 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| R-PEQH-50-5001 | 565 | 575 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg | |
| APCQH-50-5003 | 652 | 662 | 1 mg / 5 mg / 10 mg / 25 mg / 100 mg |
For support, include: Conjugation partner, coupling route, material amount and product quality requirements
R-PE, B-PE, APC and PerCP are conjugation materials here. Check R-PE and B-PE separately rather than substituting parameters under the common PE name.
Both the fluorescent material and target antibody are macromolecules; a simple desalting step does not ensure separation of the conjugate from free fluorescent protein.
These papers and manufacturer methods are selected for the current product use. Their reagents, conditions and parameters belong to the reported systems, not to measured Qihe product performance or universal operating conditions.
Thermo Fisher Scientific · Phycobiliproteins · MP 00800
Manufacturer guidance for phycobiliproteins such as R-PE/APC, distinguishing native reagents, derivatives and macromolecular conjugate purification. PerCP is not a phycobiliprotein; confirm its activation, purification and storage separately.
Jackson ImmunoResearch · Peridinin-Chlorophyll-protein, PerCP
Background on the PerCP complex and detection with antibody or streptavidin conjugates. The source concerns that manufacturer’s finished probes, not an activation recipe, optical specification or performance guarantee for Qihe PerCP material.
Cossarizza A, Chang HD, Radbruch A, et al. · European Journal of Immunology · 51(12):2708–3145 · 2021
Provides methodological guidance on detection settings, controls and experimental records for downstream conjugate evaluation.
Select a catalog number and the information you need, then enter your phone and email for a reply. Include the vial lot number for a CoA.
Choose a product above to enable the request buttons.
Product use, preparation, operating conditions and storage.
Spectra, measurement conditions and detection settings for the exact catalog number.
Lot-specific test results. Include the lot number from the vial.
Handling, storage and emergency response information.
These products supply fluorescent material. Antigen specificity comes from the antibody or recognition molecule attached later. Confirm the fluorescent protein, formulation and activation state before designing coupling and purification.
Their specific spectra differ, and preparation and conjugation parameters require catalog-specific checks. Select using actual excitation/emission and instrument configuration, then reassess signal, background and function after switching material.
First check the label and documentation for a supplied reactive handle. Pair preactivated material with that handle and avoid reactivation. For unactivated material, prepare the fluorescent protein and target handles separately, removing free activator or reductant before coupling.
Both fluorescent protein and antibody are macromolecules. Simple desalting removes some small-molecule impurities but does not ensure separation of free protein, conjugate and aggregates. Validate size-exclusion or another method that resolves those components.
Follow each formulation’s requirements. Material concentration, buffer and activation state may differ from the final antibody conjugate, so storage and freeze–thaw conditions are not automatically transferable.
We use cookies and third-party analytics (Microsoft Clarity, Baidu Tongji, Google Analytics) to measure traffic and improve our service. Clarity includes session recording. You may decline — core site functionality is unaffected. View privacy policy
